Cortical spreading depression decreases Fos expression in rat periaqueductal gray matter

NEUROSCIENCE LETTERS

卷:585

Article

Bogdanov, Volodymyr Borysovych^1,2,3,4  Bogdanova, Olena Viktorivna²  Lombard, Arnaud¹  Chauvel, Virginie¹  Multon, Sylvie¹  Kot, Larysa Ivanivna²  Makarchuk, Mykola Yukhymovych²  Schoenen, Jean¹ 

[1] Univ Liege, Headache Res Unit, B-4000 Liege, Belgium

[2] Taras Shevchenko Natl Univ Kyiv, UA-01033 Kiev, Ukraine

[3] INRA, Nutr & Neurobiol Integree, 146 Rue Leo Saignat, F-33076 Bordeaux, France

[4] Univ Bordeaux, Nutr & Neurobiol Integree, UMR 1286, F-33076 Bordeaux, France

关键词: Cortical spreading depression;    Periaqueductal gray matter;    Edinger-Westphal nucleus;    Fos expression;    Migraine;   

DOI  :  10.1016/j.neulet.2014.11.026

来源: Elsevier

【 摘 要 】

The migraine headache involves activation and central sensitization of the trigeminovascular pain pathway. The migraine aura is likely due to cortical spreading depression (CSD), a propagating wave of brief neuronal depolarization followed by prolonged inhibition. The precise link between CSD and headache remains controversial. Our objectives were to study the effect of CSD on neuronal activation in the periaqueductal grey matter (PAG), an area known to control pain and autonomic functions, and to be involved in migraine pathogenesis. Fos-immunoreactive nuclei were counted in rostral FAG and Edinger-Westphal nuclei (PAG-EWn bregma -6.5 mm), and caudal PAG (bregma -8 mm) of 17 adult male Sprague-Dawley rats after KCl-induced CSD under chloral hydrate anesthesia. Being part of a pharmacological study, six animals had received, for the preceding 4 weeks daily, intraperitoneal injections of lamotrigine (15 mg/kg), six others had been treated with saline, while five sham-operated animals served as controls. We found that the number of Fos-immunoreactive nuclei in the PAG decreased after CSD provocation. There was no difference between lamotrigine- and saline-treated animals. The number of CSDs correlated negatively with Fos-immunoreactive counts. CSD-linked inhibition of neuronal activity in the FAG might play a role in central sensitization during migraine attacks and contribute to a better understanding of the link between the aura and the headache. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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