Intrathecal L-arginine reduces the antinociception of sevoflurane in formalin-induced pain in rats

NEUROSCIENCE LETTERS

卷:590

Article

Chen, Jian-Qing^1,2  Zeng, Yin-Ming²  Dai, Ti-Jun²  Tang, Qi-Feng³ 

[1] Peoples Hosp Jiangyin, Dept Anesthesiol, Jiangyin 214400, Peoples R China

[2] XuZhou Med Coll, Jiangsu Prov Key Lab Anesthesiol, Xuzhou 221002, Peoples R China

[3] Nanjing Med Univ, Suzhou BenQ Med Ctr, Dept Anesthesiol, Suzhou 215009, Peoples R China

关键词: Sevoflurane;    Nitric oxide;    Antinociception;    L-arginine;    Pain;    c-Fos;   

DOI  :  10.1016/j.neulet.2015.02.006

来源: Elsevier

【 摘 要 】

The purpose of this study was to investigate the contribution of spinal nitric oxide (NO) to the antinociceptive effects of emulsified sevoflurane in rats. Formalin tests were used to assess the nociceptive response. Immunohistochemistry was performed to determine the effects of emulsified sevoflurane on formalin-induced changes of Fos-like immunoreactive (Fos-LI)-positive neurons in the spinal cord. We found that emulsified sevoflurane administered intraperitoneally at a dosage of 2.5 ml/kg did not impair motor performance in rats, but it significantly decreased the formalin-induced paw licking time. Furthermore, Fos-LI-positive neurons were mainly found in the ipsilateral dorsal horn after the injection of formalin. The administration of emulsified sevoflurane significantly decreased Fos-LI-labeled neurons. Finally, intrathecal L-arginine alone did not affect the basal pain threshold, but it significantly decreased the antinociceptive response of emulsified sevoflurane against formalin injection and the suppressive effects of sevoflurane on formalin-induced Fos protein expression (P< 0.05). These data suggest that spinal cord NO is involved in the antinociception of sevoflurane in rats. (c) 2015 The Authors. Published by Elsevier Ireland Ltd.

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