Sex-specific alterations in hippocampal cannabinoid 1 receptor expression following adolescent delta-9-tetrahydrocannabinol treatment in the rat

NEUROSCIENCE LETTERS

卷:602

Article

Silva, Lindsay¹  Harte-Hargrove, Lauren¹  Izenwasser, Sari²  Frank, Ashley²  Wade, Dean²  Dow-Edwards, Diana¹ 

[1] Suny Downstate Med Ctr, Dept Physiol & Pharmacol, Brooklyn, NY 11203 USA

[2] Univ Miami, Dept Psychiat & Behav Sci, Miller Sch Med, Miami, FL 33136 USA

关键词: Delta-9-Tetrahydrocannabinol;    Sex differences;    Adolescence;    CB1 receptor expression;    Hippocampus;   

DOI  :  10.1016/j.neulet.2015.06.033

来源: Elsevier

【 摘 要 】

Marijuana use by adolescents has been on the rise since the early 1990s. With recent legalization and decriminalization acts passed, cannabinoid exposure in adolescents will undoubtedly increase. Human studies are limited in their ability to examine underlying changes in brain biochemistry making rodent models valuable. Studies in adult and adolescent animals show region and sex specific downregulation of the cannabinoid 1 (CBI) receptor following chronic cannabinoid treatment. However, although sex-dependent changes in behavior have been observed during the drug abstinence period following adolescent cannabinoid exposure, little is known about CBI receptor expression during this critical time. In order to characterize CBI receptor expression following chronic adolescent Delta-9-tetrahydrocannabinol (THC) exposure, we used [H-3] CP55,940 binding to assess CBI receptor expression in the dentate gyrus and areas CA1, CA2, and CA3 of the hippocampus in both male and female adolescent rats at both 24 hand 2 weeks post chronic THC treatment. Consistent with other reported findings, we found downregulation of the CBI receptor in the hippocampal formation at 24 h post treatment. While this downregulation persisted in both sexes following two weeks of abstinence in the CA2 region, in females, this downregulation also persisted in areas CM and CA3. Expression in the dentate gyrus returned to the normal range by two weeks. These data suggest that selective regions of the hippocampus show persistent reductions in CBI receptor expression and that these reductions are more widespread in female compared to male adolescents. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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