Sevoflurane induced amnesia inhibits hippocampal Arc expression partially through 5-hydroxytryptamine-7 receptors in the bilateral basolateral amygdala in rats

NEUROSCIENCE LETTERS

卷:562

Article

Zhang, Fujun¹  Feng, Xiaomei¹  Zeng, Qingwen²  Wang, Bo²  Wilhelmsen, Kevin³  Li, Qiang¹  Cao, Xiaohua²  Yu, Buwei¹ 

[1] Shanghai Jiao Tong Univ, Sch Med, Ruijin Hosp, Dept Anesthesiol, Shanghai 200025, Peoples R China

[2] E China Normal Univ, Key Lab Brain Funct Genom, Minist Educ, Shanghai Key Lab Brain Funct Genom, Shanghai 200062, Peoples R China

[3] Univ Calif San Francisco, San Francisco Gen Hosp, Dept Anesthesia & Perioperat Care, San Francisco, CA 94143 USA

关键词: Sevoflurane;    Basolateral amygdala;    Learning and memory;    5-Hydroxytryptamine receptor;    Activity-regulated cytoskeletal protein;    Neural apoptosis;   

DOI  :  10.1016/j.neulet.2013.12.066

来源: Elsevier

【 摘 要 】

This study aimed to investigate whether the regulation of 5-hydroxytryptamine-7 (5-HT7) receptors in the bilateral basolateral amygdala (BLA) could alter the amnesic effects of sevoflurane and change the hippocampal expression of Arc and neural apoptosis. Male Sprague-Dawley rats were randomized into ten groups. First, the animals received bilateral injection of SB269970 (20, 50, or 100 pmol/0.2 mu l) or saline (0.2 mu l) or AS-19 (2, 10, or 50 pmol/0.2 mu l), followed by inhalation of 2% sevoflurane or air for 2h. Then, fear conditioning training was carried out, and the percentage of freezing was detected 2411 later. Furthermore, hippocampal Arc protein level and neural apoptosis were measured. Pre-training inhalation of sevoflurane reduced the extent of freezing, and hippocampal Arc expression. The largest dose of SB269970 (100 pmol) could block sevoflurane-induced amnesia and reverse the inhibitive effect of sevoflurane on Arc expression, while the maximal dose of AS-19 could exacerbate the amnesic effect, and further inhibit Arc expression. Furthermore, pre-training inhalation of 2% sevoflurane for 6h could not induce neural apoptosis in the hippocampus. The amnesic effect of sevoflurane might partly attribute to its impairment of memory formation in the hippocampus via activation of 5-HT7 receptors in the BLA. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

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