Anti-prion activity generated by a novel vaccine formulation

NEUROSCIENCE LETTERS

卷:429

Article

Pilon, John¹  Loiacono, Christina²  Okeson, Danelle¹  Lund, Sharon²  Vercauteren, Kurt¹  Rhyan, Jack³  Miller, Lowell¹ 

[1] USDA APHIS WS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA

[2] USDA APHIS, Natl Vet Serv Labs, Ames, IA 50010 USA

[3] USDA APHIS VS, Natl Wildlife Res Ctr, Ft Collins, CO 80521 USA

关键词: prion;    chronic wasting disease (CWD);    transmissible spongiform encephalopathy (TSE);    scrapie;    vaccine;   

DOI  :  10.1016/j.neulet.2007.10.015

来源: Elsevier

【 摘 要 】

Chronic wasting disease (CWD) is a transmissible spongiform encephalopathy (TSE) of domestic and wild cervids in North America. To address possible prevention regimens for CWD, we have used a mouse model system and the Rocky Mountain Laboratory (RML) mouse-adapted scrapie prion strain to screen efficacy of potential vaccine candidates. Three peptides derived from the primary amino acid sequence of the prion protein were conjugated to blue carrier protein (BCP) and formulated in an adjuvant containing M. avium subsp. avium. CL57/BL6 mice were vaccinated and boosted with 50 mu g of the carrier protein-peptide conjugate formulation; all vaccines produced a humoral immune response as measured by ELISA. Disease challenge with the RML scrapie prion strain revealed anti-prion activity was generated by the vaccine formulations as measured by a delay in clinical disease onset and prolonged survivorship. (C) 2007 Elsevier Ireland Ltd. All rights reserved.

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