Gait analysis of spinal cord injured rats after delivery of chondroitinase ABC and adult olfactory mucosa progenitor cell transplantation

NEUROSCIENCE LETTERS

卷:472

Article

Huang, Wen-Cheng^2,4,5,6  Hsu, Sung-Hao⁷  Cheng, Chu-Hsun^4,5  Liu, Jiang-Chuan³  Cheng, Henrich^1,2,4,5,6 

[1] Taipei Vet Gen Hosp, Dept Neurosurg, Neural Regenerat Lab, Ctr Neural Regenerat,Neurol Inst, Taipei 11217, Taiwan

[2] Natl Def Med Ctr, Grad Inst Med Sci, Taipei, Taiwan

[3] Natl Def Med Ctr, Inst Biol & Anat, Taipei, Taiwan

[4] Natl Yang Ming Univ, Sch Med, Dept Pharmacol, Taipei 112, Taiwan

[5] Natl Yang Ming Univ, Sch Med, Inst Pharmacol, Taipei 112, Taiwan

[6] Natl Yang Ming Univ, Sch Med, Fac Med, Taipei 112, Taiwan

[7] Natl Taiwan Univ, Coll Life Sci, Inst Zool, Taipei, Taiwan

关键词: Spinal cord injury (SCI);    Chondroitin sulfate proteoglycan;    Chondroitinase ABC;    Intrathecal catheter;    Olfactory mucosa progenitor cells;    Treadmil;   

DOI  :  10.1016/j.neulet.2010.01.012

来源: Elsevier

【 摘 要 】

Chondroitin sulfate proteoglycan (CSPG) is a major component of glial scar to restrict axonal regeneration in the lesion site after spinal cord injury (SCI). Chondroitinase ABC (ChABC), a bacteria enzyme, which has been demonstrated to digest the glycosaminoglycan (GAG) side chain of CSPG to promote axonal re-growth across the injured site. Our previous study suggested that long-term delivery of ChABC (1 U/ml, injection volume 0.6 mu l for one animal) via intrathecal catheter could decrease the inhibitory effect of limiting axonal re-growth after SCI. The functional behavior has been shown to improve following ChABC treatment. Little axons re-grow across the lesion site of the spinal cord but not enough to support axon innervations to targets. In this article, we show that ChABC administration combining olfactory mucosa progenitor cell (OMPC) transplantation can promote axonal re-growth across the lesion site and enhance the consistency of stepping in spinally transected rats. These OMPCs generated NG2(+) cell lineages after transplanting into the spinal cord parenchyma, and OMPCs were found to spread and migrate toward the lesion region of spinal cord. Moreover, the spatial and temporal characteristics of the step cycle in rats that receive a complete spinal cord transaction following continuous ChABC supply and OMPC transplantation. The gait characteristics of treated rats on a treadmill were consistent and approached that of intact rats. In future, the mechanism of restoring the injured spinal cord will be further investigated. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

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