Multiple roles for the first transmembrane domain of GABAA receptor subunits in neurosteroid modulation and spontaneous channel activity

NEUROSCIENCE LETTERS

卷:473

Article

Baker, Carrie²  Sturt, Brianne L.¹  Bamber, Bruce A.¹ 

[1] Univ Toledo, Dept Biol Sci, Toledo, OH 43606 USA

[2] Univ Toledo, Dept Bioengn, Toledo, OH 43606 USA

关键词: GABA(A) receptor;    Neurosteroid;    PS;    THDOC;    Direct activation;    Spontaneous opening;   

DOI  :  10.1016/j.neulet.2010.02.058

来源: Elsevier

【 摘 要 】

Neurosteroids exert potent physiological effects by allosterically modulating synaptic and extrasynaptic GABA(A) receptors. Some endogenous neurosteroids, such as 3 alpha, 21-dihydroxy-5 beta-pregnan-20-one (5 alpha, 3 alpha-THDOC), potentiate GABA(A) receptor function by interacting with a binding pocket defined by conserved residues in the first and fourth transmembrane (TM) domains of alpha subunits. Others, such as pregnenolone sulfate (PS), inhibit GABA(A) receptor function through as-yet unidentified binding sites. Here we investigate the mechanisms of PS inhibition of mammalian GABA(A) receptors, based on studies of PS inhibition of the UNC-49 GABA receptor, a GABA(A)-like receptor from Caenorhabditis elegans. In UNC-49, a 19 residue segment of TM1 can be mutated to increase or decrease PS sensitivity over a 20-fold range. Surprisingly, substituting these UNC-49 sequences into mammalian alpha(1), beta(2), and gamma(2) subunits did not produce the corresponding effects on PS sensitivity of the resulting chimeric receptors. Therefore, it is unlikely that a conserved PS binding pocket is formed at this site. However we observed several interesting unexpected effects. First, chimeric gamma(2) subunits caused increased efficacy of 5 alpha, 3 alpha-THDOC potentiation; second, spontaneous gating of alpha(6)beta(2)delta receptors was blocked by PS, and reduced by chimeric beta(2) subunits; and third, direct activation of alpha(6)beta(2)delta receptors by 5 alpha, 3 alpha-THDOC was reduced by chimeric beta(2) subunits. These results reveal novel roles for non-alpha subunits in neurosteroid modulation and direct activation, and show that the beta subunit TM1 domain is important for spontaneous activity of extrasynaptic GABAA receptors. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free