Thrombin preferentially induces autophagy in glia cells in the rat central nervous system

NEUROSCIENCE LETTERS

卷:630

Article

Hu, Shukun¹  Wu, Gang¹  Ding, Xin²  Zhang, Yi¹ 

[1] Fudan Univ, Affiliated Huashan Hosp, Dept Neurosurg, 12 Wulumuqi Middle Rd, Shanghai 200042, Peoples R China

[2] Soochow Univ, Affiliated Childrens Hosp, Div Neonatol, Suzhou 215003, Peoples R China

关键词: Intracerebral hemorrhage;    Autophagy;    Thrombin;    Astrocyte;    Neuron;   

DOI  :  10.1016/j.neulet.2016.07.023

来源: Elsevier

【 摘 要 】

Autophagy widely occurs after intracerebral hemorrhage (ICH). In our previous study, we demonstrated that thrombin, a serine protease produced after hematoma, contributes to ICH-induced autophagy. However, whether thrombin plays a neuronal and/or astrocytic role in autophagy induction is largely unknown. Here, we examined the autophagic role of thrombin on neurons and glia cells, respectively. In vivo, we found that intracaudate injection of thrombin specifically elevated the astrocytic expression of beclin-1 and LC3, two autophagic markers, and promoted the formation of autophagic vacuoles within astrocytes rather than neurons in the ipsilateral basal ganglia. Consistent with this, thrombin enhanced the LC3-II level and increased the number of MDC-Iabeled autophagic vacuoles in cultured astrocytes. These results indicated that thrombin preferentially activated astrocytic autophagy after ICH, and therefore provided novel insights into the pathophysiological mechanisms and therapeutic targets for hemorrhage stroke and brain trauma. (C) 2016 Published by Elsevier Ireland Ltd.

【 授权许可】

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