Neocortical integration of transplanted GABA progenitor cells from wild type and GABAB receptor knockout mouse donors

NEUROSCIENCE LETTERS

卷:561

Article

Sebe, Joy Y.¹  Looke-Stewart, Elizabeth¹  Dinday, Matthew T.¹  Alvarez-Buylla, Arturo^1,2,3  Baraban, Scott C.^1,2,3 

[1] Univ Calif San Francisco, Dept Neurol Surg, San Francisco, CA 94143 USA

[2] Univ Calif San Francisco, Grad Program Neurosci, San Francisco, CA 94143 USA

[3] Univ Calif San Francisco, Dept Neurol Surg, Eli & Edythe Broad Ctr Regenerat Med & Stem Cell, San Francisco, CA 94143 USA

关键词: Interneuron;    GABA;    Laminar;    Neocortex;    Transplantation;   

DOI  :  10.1016/j.neulet.2013.11.012

来源: Elsevier

【 摘 要 】

Most cortical interneurons originate in a region of the embryonic subpallium called the medial ganglionic eminence (MGE). When MGE cells are transplanted into cerebral cortex, these progenitors migrate extensively and differentiate into functional inhibitory neurons. Although MGE progenitors have therapeutic potential following transplantation, it is unknown precisely how these cells distribute within neocortical lamina of the recipient brain. Here we transplanted mouse embryonic day 12.5 MGE progenitors into postnatal neocortex and evaluated laminar distribution of intemeuron subtypes using double- and triple-label immunohistochemistry. Studies were performed using wild type (WT) or donor mice lacking a metabotropic GABA(B) receptor subunit (GABA(B1) R KO). MGE-derived neurons from WT and GABA(B1) R KO mice preferentially and densely distributed in neocortical layers 2/3,5 and 6. As expected, MGE-derived neurons differentiated into parvalbumin+ and somatostatin+ intemeurons within these neocortical lamina. Our findings provide insights into the anatomical integration of MGE-derived interneurons following transplantation. (C) 2013 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free