Transduction of an immortalized olfactory ensheathing glia cell line with the green fluorescent protein (GFP) gene: Evaluation of its neuroregenerative capacity as a proof of concept

NEUROSCIENCE LETTERS

卷:612

Article

Plaza, N.¹  Simon, D.¹  Sierra, J.¹  Moreno-Flores, M. T.¹ 

[1] Univ Francisco Vitoria, Dept Biotechnol, Neuroregenerat Grp, Ctra Pozuelo Majadahonda,Km 1-800, Madrid 28223, Spain

关键词: Immortalized human olfactory ensheathing glia (ihOEG);    Retinal ganglion neuron (RGN);    Axonal regeneration;    Green fluorescent protein (GFP);    Proof of concept;   

DOI  :  10.1016/j.neulet.2015.12.001

来源: Elsevier

【 摘 要 】

Olfactory ensheathing glia (OEG) cells are known to foster axonal regeneration of central nervous system (CNS) neurons. Several lines of reversibly immortalized human OEG (ihOEG) have been previously established that enabled to develop models for their validation in vitro and in vivo. In this work, a constitutively GFP-expressing ihOEG cell line was obtained, and named Ts14-GFP. Ts14-GFP neuroregenerative ability was similar to that found for the parental line Ts14 and it can be assayed using in vivo transplantation experimental paradigms, after spinal cord or optic nerve damage. Additionally, we have engineered a low-regenerative ihOEG line, hTL2, using lentiviral transduction of the large T antigen from SV40 virus, denominated from now on Ts12. Ts12 can be used as a low regeneration control in these experiments. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free