Chronic restraint stress in rats suppresses sweet and umami taste responses and lingual expression of T1R3 mRNA

NEUROSCIENCE LETTERS

卷:486

Article

Okamoto, Atsuhiro^1,2  Miyoshi, Michio¹  Imoto, Toshiaki¹  Ryoke, Kazuo²  Watanabe, Tatsuo¹ 

[1] Tottori Univ, Fac Med, Dept Funct Morphol & Regulatory Sci, Div Integrat Physiol, Yonago, Tottori 683, Japan

[2] Tottori Univ, Fac Med, Dept Med Sensory & Motor Organs, Div Oral & Maxillofacial Biopathol Surg, Yonago, Tottori 683, Japan

关键词: Stress;    Taste disorder;    Sweet taste;    Umami taste;    T1R3 mRNA;    Tongue;   

DOI  :  10.1016/j.neulet.2010.09.055

来源: Elsevier

【 摘 要 】

Effects of chronic restraint stress on the taste responses to five basic taste qualities were investigated electrophysiologically in the rat chorda tympani In addition the mRNA expression for T1R3 the common G-protein-coupled receptor (GPCR) for sweet and umami tastes was studied quantitatively by RT-PCR after such stress Rats were restrained in a small cylindrical restrainer made of steel wire for 8 h daily for 14 successive days The integrated responses to sweet and umami tastes as recorded from the chorda tympani were significantly suppressed after such stress but the other three basic taste responses were unaffected Expression of T1R3 mRNA in the fungiform papillae as estimated by RT-PCR was slightly reduced by the stress and a quantitative real time RT-PCR study revealed a significant suppression of T1R3 mRNA expression in the stress group These results suggest that the observed stress-Induced changes in taste sensation could be caused by a peripheral disorder of the transduction mechanism in taste-receptor cells involving in particular a stress-induced inhibition of T1R3 expression (C) 2010 Elsevier Ireland Ltd All rights reserved

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