Enhancement of tonic and phasic GABAergic currents following nitric oxide synthase inhibition in hippocampal CA1 pyramidal neurons

NEUROSCIENCE LETTERS

卷:590

Article

Gasulla, Javier¹  Calvo, Daniel J.¹ 

[1] Consejo Nacl Invest Cient & Tecn, Lab Neurobiol Celular & Mol, Inst Invest Ingn Genet & Biol Mol INGEBI Dr Hecto, RA-1428 Buenos Aires, DF, Argentina

关键词: GABA(A) receptors;    Tonic inhibition;    Phasic inhibition;    Nitric oxide synthase;    Hippocampus;   

DOI  :  10.1016/j.neulet.2015.01.058

来源: Elsevier

【 摘 要 】

Nitric oxide (NO) is involved in synaptic plasticity in the hippocampus through different presynaptic and postsynaptic mechanisms that include the modulation of the GABAergic neurotransmission. Inhibitory synapses on hippocampal pyramidal neurons are known to possess the molecular machinery for retrograde NO-signaling, but the modulation of GABA(A)Rs function by NO in these neurons and the mechanisms of action involved have not been fully characterized. Here we show that suppression of the endogenous NO generation by the nitric oxide synthase (NOS) inhibitor L-NAME produces significant and reversible increases in the magnitude of both tonic and phasic GABAergic currents in CA1 hippocampal pyramidal neurons. GABA-evoked chloride currents were measured in the presence or absence of L-NAME using whole-cell patch-clamp recordings in acute hippocampal slices from young adult mice. Enhancement of the tonic GABA responses induced by L-NAME was insensitive to TTX and decreased by co-incubation with the NO donor DEA/NO. Applications of DEA/NO alone did not produce significant effects on tonic GABA responses. L-NAME treatment also increased the amplitude of phasic GABAergic currents evoked by GABA-puffs. Our results indicate that the extent of tonic and phasic inhibition mediated by GABA(A) receptors in CA1 hippocampal pyramidal neurons is affected by endogenous NO production. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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