Calcineurin inhibition with systemic FK506 treatment increases dendritic branching and dendritic spine density in healthy adult mouse brain

NEUROSCIENCE LETTERS

卷:487

Article

Spires-Jones, Tara L.¹  Kay, Kevin¹  Matsouka, Roland²  Rozkalne, Anete¹  Betensky, Rebecca A.²  Hyman, Bradley T.¹ 

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, MassGen Inst Neurodegenerat Dis, Charlestown, MA 02129 USA

[2] Harvard Univ, Sch Publ Hlth, Boston, MA 02115 USA

关键词: Tacrolimus;    FK506;    Dendritic spine;    Dendrite branching;    Sholl plot;    Survival analysis;   

DOI  :  10.1016/j.neulet.2010.10.033

来源: Elsevier

【 摘 要 】

Calcineurin has been implicated as part of a critical signaling pathway for learning and memory, and recent data suggest that calcineurin activation mediates some of the neurotoxicity of the Alzheimer related neurotoxin A beta. Immunosuppression via calcineurin inhibition with the compound FK506 is an important treatment for organ transplant patients. Here we use Golgi impregnation techniques, along with a new survival analysis-based statistical approach for analysis of dendritic complexity, to show that in healthy adult mice one week of treatment with FK506 affects both the branching patterns and dendritic spine density of cortical neurons. These results indicate that calcineurin inhibition leads to readily detectable changes in brain morphology, further implicating calcineurin related pathways in both the function and structure of the adult brain. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free