A new method to effectively and rapidly generate neurons from SH-SY5Y cells

NEUROSCIENCE LETTERS

卷:610

Article

Yang, HongNa^1,2  Wang, Jing¹  Sun, JinHua²  Liu, XiaoDun³  Duan, Wei-Ming⁴  Qu, TingYu² 

[1] Shandong Univ, Qilu Hosp, Dept Crit Care Med, Jinan 250012, Shandong, Peoples R China

[2] Univ Illinois, Coll Med, Dept Psychiat, Chicago, IL 60612 USA

[3] R&D Cell & Tissue Bank Shandong Prov, Jinan 250000, Shandong, Peoples R China

[4] Capital Med Univ, Dept Anat, Beijing 100069, Peoples R China

关键词: SH-SY5Y cells;    RA;    CM-hNSCs;    Neurons;    beta-tubulin III;   

DOI  :  10.1016/j.neulet.2015.10.047

来源: Elsevier

【 摘 要 】

It is well known that neurons differentiated from SH-SY5Y cells can serve as cell models for neuroscience research; i.e., neurotoxicity and tolerance to morphine in vitro. To differentiate SH-SY5Y cells into neurons, RA (retinoic acid) is commonly used to produce the inductive effect. However, the percentage of neuronal cells produced from SH-SY5Y cells is low, either from the use of RA treatment alone or from the combined application of RA and other chemicals. In the current study, we used CM-hNSCs (conditioned medium of human neural stem cells) as the combinational inducer with RA to prompt neuronal differentiation of SH-SY5Y cells. We found that neuronal differentiation was improved and that neurons were greatly increased in the differentiated SH-SY5Y cells using a combined treatment of CM-hNSCs and RA compared to RA treatment alone. The neuronal percentage was higher than 80% (about 88%) on the 3rd day and about 91% on the 7th day examined after a combined treatment with CM-hNSCs and RA. Cell maturation and neurite growth of these neuronal cells were also improved. In addition, the use of CM-hNSCs inhibited the apoptosis of RA-treated SH-SY5Y cells in culture. We are the first to report the use of CM-hNSCs in combination with RA to induce neuronal differentiation of RA-treated SH-SY5Y cells. Our method can rapidly and effectively promote the neuronal production of SH-SY5Y cells in culture conditions. (C) 2015 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

Free