Nitric oxide stimulates glutamatergic synaptic inputs to baroreceptor neurons through potentiation of Cav2.2-mediated Ca2+ currents

NEUROSCIENCE LETTERS

卷:567

Article

Li, De-Pei¹  Chen, Shao-Rui² 

[1] Univ Texas MD Anderson Canc Ctr, Dept Crit Care, Houston, TX 77030 USA

[2] Univ Texas MD Anderson Canc Ctr, Dept Anesthesiol & Perioperat Med, Houston, TX 77030 USA

关键词: Nitric oxide;    Glutamate;    Baroreceptor;    Ca2+ channels;    Brainstem;   

DOI  :  10.1016/j.neulet.2014.03.036

来源: Elsevier

【 摘 要 】

Nitric oxide (NO) increases glutamate release to the second-order neurons in the nucleus tractus solitarius (NTS). N-type Ca2+ channel is essential for triggering glutamate release at synaptic terminals. In this study, we determined the role of Ca(v)2.2 subunit in NO-induced increase in glutamate synaptic inputs to NTS neurons. The second-order NTS neurons and nodose ganglionic (NG) neurons were identified by applying DiA, a fluorescent lipophilic tracer, on aortic depressor nerve in rats. NO donor DEA/NO significantly increased tractus solitarius (TS)-evoked excitatory postsynaptic currents (EPSCs) in second-order NTS neurons, an effect was abolished by pretreatment of slice with ODQ an inhibitor for soluble isoform of guanylyl cyclase. DEA/NO decreased the paired-pulse ratio of TS-evoked EPSCs, while increased the frequency, but not the amplitude, of miniature EPSCs in second-order NTS neurons. Furthermore, DEA/NO significantly increased Ba2+ currents in identified baroreceptor NG neurons. However, DEA/NO had little effect on the Ba2+ currents in the presence of specific N-type Ca2+ blocker omega-conotoxin GVIA. In addition, immunocytochemistry staining revealed that Ca(v)2.2 subunit immunoreactivates were colocalized with DiA-labeled baroreceptor nerve terminals in the NTS. Collectively, these findings suggest that NO stimulates glutamatergic synaptic inputs to second-order NTS neurons through augmentation of Ca(v)2.2-mediated N-type Ca2+ currents. (C) 2014 Elsevier Ireland Ltd. All rights reserved.

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