Contribution of P2Y1 receptors to ADP signalling in mouse spinal cord cultures

NEUROSCIENCE LETTERS

卷:435

Article

Atterbury-Thomas, Amelia E.¹  Leon, Catherine²  Gachet, Christian²  Forsythe, Ian D.¹  Evans, Richard J.¹ 

[1] Univ Leicester, Dept Cell Physiol & Pharmacol, Leicester LE1 9HN, Leics, England

[2] EFS, INSERM, U311, F-67065 Strasbourg, France

关键词: P2Y(1);    P2Y(12);    P2Y(13);    spinal cord;    calcium;    phosphorylation;    neurons;    glia;    ADP;   

DOI  :  10.1016/j.neulet.2008.02.034

来源: Elsevier

【 摘 要 】

Mixed neuronal and glial cell spinal cord cultures from neonates express ADP sensitive P2Y(1,12&13) receptors. ADP (10 mu M) evoked increases in intracellular calcium that were essentially abolished by the P2Y(1) receptor antagonist MRS2179 (10 mu M), responses were also absent in preparations from P2Y1 receptor deficient mice however UTP(100 mu M) evoked calcium rises were unaffected. ADP also evoked a robust increase in extracellular signal-regulated protein kinase (ERK) phosphorylation that was of similar magnitude in the cultures from wild type and P2Y1 receptor deficient mice. These results suggest that ADP acts through P2Y(1) receptors to mediate an increase in intracellular calcium but not to stimulate ERK phosphorylation in the spinal cord. (c) 2008 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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