Distribution of AMPA receptor subunits and TARPs in synaptic and extrasynaptic membranes of the adult rat nucleus accumbens

NEUROSCIENCE LETTERS

卷:490

Article

Ferrario, Carrie R.¹  Loweth, Jessica A.¹  Milovanovic, Mike¹  Wang, Xiaoting¹  Wolf, Marina E.¹ 

[1] Rosalind Franklin Univ Med & Sci, Dept Neurosci, N Chicago, IL 60064 USA

关键词: Addiction;    Ca2+-permeable AMPA receptor;    NMDA receptor;    Plasticity;    Subcellular fractionation;    TARP;   

DOI  :  10.1016/j.neulet.2010.12.036

来源: Elsevier

【 摘 要 】

We characterized the distribution of AMPA receptor (AMPAR) subunits and the transmembrane AMPA receptor regulatory proteins (TARPs) gamma-2 and gamma-4 in adult rat nucleus accumbens (NAc) using a method that separates plasma membranes into synaptic membrane-enriched and extrasynaptic membrane-enriched fractions. We also measured GluA1 phosphorylated at serine 845 (pS845 GluA1) and serine 831 (pS831 GluA1). GluA1-3 protein levels and pS831 GluA1/total GluA1 were higher in synaptic membranes. However, pS845 GluA1/total GluA1 was higher in extrasynaptic membranes, consistent with a role for 5845 phosphorylation in GluA 1 insertion at extrasynaptic sites. Homeric GluA1 receptors were detected in extrasynaptic membranes, consistent with evidence for extrasynaptic Ca2+-permeable AMPARs in other systems. The TARP gamma-2 was enriched in synaptic membranes, whereas gamma-4 was mainly found in extrasynaptic membranes, suggesting distinct roles for these proteins in the NAc. These experiments provide fundamental information that will aid in the interpretation of studies on AMPAR-related plasticity in the NAc. (C) 2010 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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