Differential expression of cocaine- and amphetamine-regulated transcript-immunoreactivity in the rat spinal preganglionic nuclei

NEUROSCIENCE LETTERS

卷:294

Article

Dun, SL ; Chianca, DA ; Dun, NJ ; Yang, J ; Chang, JK

关键词: intermediolateral cell column;    parasympathetic preganglionic neurons;    sympathetic preganglionic neurons;    spinal cord;   

DOI  :  10.1016/S0304-3940(00)01575-5

来源: Elsevier

【 摘 要 】

The distribution of cocaine- and amphetamine-regulated transcript-like immunoreactivity (CART-LI) was investigated in the rat spinal cords with the use of an antiserum against the CART peptide fragment 55-102. CART-LI fibers were concentrated in the superficial layers of the dorsal horn of all segments. In addition to CART-LI fibers, intensely labeled somata were detected in the intermediolateral cell column (IML) and other sympathetic preganglionic nuclei of the thoracolumbar segments. In the lumbosacral segments, CART-LI fibers but not somata were seen in the sacral parasympathetic nucleus. Double-labeling the spinal sections with choline acety]transferase (ChAT)-antisera and CART-antisera revealed that the large majority of ChAT-positive somata in the sympathetic preganglionic nuclei were CART-positive, whereas ChAT-positive somata in the parasympathetic preganglionic nuclei were CART-negative. Our results show that CART-LI is selectively expressed in a population of sympathetic preganglionic neurons (SPNs), but not in parasympathetic preganglionic neurons (PPNs) of the rat. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.

【 授权许可】

Free