Analysis of metabotropic glutamate receptor 7 as a potential substrate for SUMOylation

NEUROSCIENCE LETTERS

卷:491

Article

Wilkinson, Kevin A.¹  Henley, Jeremy M.¹ 

[1] Univ Bristol, Sch Biochem, MRC, Ctr Synapt Plast, Bristol BS8 1TD, Avon, England

关键词: SUMO;    SUMOylation;    mGluR7;    Ubc9;    Ubiquitin;    Post-translational modification;    GPCR;    mGluR7;    Metabotropic glutamate receptor;   

DOI  :  10.1016/j.neulet.2011.01.032

来源: Elsevier

【 摘 要 】

Group III metabotropic glutamate receptors (mGluRs) undergo post-translational modification by SUMO in in vitro assays but the SUMOylation of full-length mGluRs in mammalian cells has not been reported. Here we investigated SUMOylation of mGluR7 in HEK293 cells and primary cortical neurons in an attempt to confirm SUMOylation and define physiological effects on mGluR7 function. Using a recombinant bacterial expression assay we validated in vitro SUMOylation of the C-terminal domain of mGluR7 by both SUMO-1 and SUMO-2 and show that a single lysine residue (K889) in mGluR7 is required for SUMOylation. However, using a range of approaches, we were unable to detect SUMOylation of full-length mGluR7 in either heterologous cells or neurons. Further, we observed no differences in receptor stability or surface expression between wild-type and a non-SUMOylatable point mutant mGluR7. Thus, our results question whether mGluR7, and by implication other group III mGluRs, are physiologically relevant neuronal SUMO substrates. (C) 2011 Elsevier Ireland Ltd. All rights reserved.

【 授权许可】

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