Altered protein expression pattern in skin fibroblasts from parkin-mutant early-onset Parkinson's disease patients

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1852

Article

Lippolis, Rosa¹  Siciliano, Rosa Anna²  Pacelli, Consiglia³  Ferretta, Anna⁴  Mazzeo, Maria Fiorella²  Scacco, Salvatore⁴  Papa, Francesco⁴  Gaballo, Antonio⁵  Dell'Aquila, Claudia⁶  De Mari, Michele⁶  Papa, Sergio¹  Cocco, Tiziana⁴ 

[1] Italian Natl Res Council CNR, Inst Biomembranes & Bioenerget, Bari, Italy

[2] Italian Natl Res Council CNR, Inst Food Sci, Avellino, Italy

[3] Univ Montreal, Fac Med, Dept Pharmacol, Montreal, PQ H3T 1J4, Canada

[4] Univ A Moro, Dept Basic Med Sci Neurosci & Sense Organs, Bari, Italy

[5] CNR, NANOTEC Ist Nanotecnol, I-73100 Lecce, Italy

[6] Bonomo Hosp, Neurol Unit, Andria, BA, Italy

关键词: Parkinson's disease;    Human skin fibroblasts;    Two-dimensional gel electrophoresis;    Mass spectrometry;    Proteomics;   

DOI  :  10.1016/j.bbadis.2015.06.015

来源: Elsevier

【 摘 要 】

Parkinson's disease (PD) is the most common neurodegenerative movement disorder caused primarily by selective degeneration of the dopaminergic neurons in substantia nigra. In this work the proteomes extracted from primary fibroblasts of two unrelated, hereditary cases of PD patients, with different parkin mutations, were compared with the proteomes extracted from commercial adult normal human dermal fibroblasts (NHDF) and primary fibroblasts from the healthy mother of one of the two patients. The results show that the fibroblasts from the two different cases of parkin-mutant patients display analogous alterations in the expression level of proteins involved in different cellular functions, like cytoskeleton structure-dynamics, calcium homeostasis, oxidative stress response, protein and RNA processing. (C) 2015 Published by Elsevier B.V.

【 授权许可】

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