Dietary zinc restriction promotes degeneration of the endocrine pancreas in mice

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1866

Article

Sisnande, Thayna¹  Lima, Cleverton K.¹  da Silva, Dayana Cabral¹  Beninatto, Thayana Moulin¹  Alves, Natalia Leao¹  Amaral, Mariana J.²  Miranda-Alves, Leandro³  Lima, Luis Mauricio T. R.^1,4 

[1] Univ Fed Rio de Janeiro, Fac Pharm, Lab Pharmaceut Biotechnol Pbiotech, BR-21941902 Rio De Janeiro, RJ, Brazil

[2] Fed Univ Rio de Janeiro UFRJ, Hosp Univ Clementino Fraga Filho, CCS, BR-21941902 Rio De Janeiro, RJ, Brazil

[3] Univ Fed Rio de Janeiro, Inst Biomed Sci, Lab Expt Endocrinol, BR-21941902 Rio De Janeiro, RJ, Brazil

[4] Natl Inst Metrol Qual & Technol INMETRO, Lab Macromol LAMAC DIMAV, Ave Nossa Senhora Gracas,50 Xerem, BR-25250020 Rio De Janeiro, RJ, Brazil

关键词: Zinc;    Pancreas;    Diabetes;    Amylin;    Insulin;    Oligomer;   

DOI  :  10.1016/j.bbadis.2020.165675

来源: Elsevier

【 摘 要 】

Zinc is a key component of several proteins, interacting with the pancreatic hormones insulin and amylin. The role of zinc in insulin oligomerization and crystallinity is well established, although the effects of dietary zinc restriction on both energetic metabolism and beta-pancreatic hormonemia and morphology remain unexplored. Here we report the effects of dietary zinc restriction on the endocrine pancreas and metabolic phenotype of mice. Nontransgenic male Swiss mice were fed a low-zinc or control diet for 4 weeks after weanling. Growth, glycemia, insulinemia and amylinemia were lower and pancreatic islets were smaller in the intervention group despite the preserved insulin crystallinity in secretory granules. We found strong immunostaining for insulin, amylin and oligomers in apoptotic pancreatic islet. High production of beta-pancreatic hormones in zinc-restricted animals counteracted the reduced islet size caused by apoptosis. These data suggest that zinc deficiency is sufficient to promote islet beta-cell hormonal disruption and degeneration.

【 授权许可】

Free