ENZYMATIC AMPLIFICATION AND MOLECULAR-CLONING OF CDNA-ENCODING THE SMALL AND LARGE SUBUNITS OF BOVINE INTERLEUKIN-12

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1270

Note

关键词: CYTOKINE;    CLONING;    INTERLEUKIN;    IL-12;    POLYMERASE CHAIN REACTION;    NUCLEOTIDE SEQUENCE;    PROTEIN SEQUENCE;   

DOI  :  10.1016/0925-4439(95)00042-3

来源: Elsevier

【 摘 要 】

cDNA generated from stimulated abomasal lymph node cells was used to amplify and clone the 35 kDa and 40 kDa subunits of bovine interleukin 12 (IL-12) using primers derived from semi-conserved regions between human and mouse IL-12 sequences. The deduced amino acid sequence of the 40 kDa subunit demonstrated 84.4% and 67.6% homology with human and mouse sequences, respectively. The deduced sequence of the 35 kDa subunit exhibited comparable similarities to the human 35 kDa subunit (82.2%) but differed significantly (58.6%) from mouse-derived sequences.

【 授权许可】

Free