Raman imaging-based phenotyping of murine primary endothelial cells to identify disease-associated biochemical alterations

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1867

Article

Tott, Szymon^1,2  Grosicki, Marek²  Glowacz, Jacek¹  Mohaissen, Tasnim^2,3  Wojnar-Lason, Kamila^2,4  Chlopicki, Stefan^2,4  Baranska, Malgorzata^1,2 

[1] Jagiellonian Univ, Fac Chem, Gronostajowa 2, PL-30387 Krakow, Poland

[2] Jagiellonian Univ, Jagiellonian Ctr Expt Therapeut JCET, Bobrzynskiego 14, PL-30348 Krakow, Poland

[3] Jagiellonian Univ, Fac Pharm, Med Coll, Medyczna 9, PL-30688 Krakow, Poland

[4] Jagiellonian Univ, Dept Pharmacol, Med Coll, Grzegorzecka 16, PL-31531 Krakow, Poland

关键词: Endothelial cells;    Raman spectroscopy;    Heart;    Aorta;    Brain;    Liver;    Kidney;   

DOI  :  10.1016/j.bbadis.2021.166180

来源: Elsevier

【 摘 要 】

Raman spectroscopy is successfully becoming an analytical tool used to characterize alterations in the biochemical composition of cells. In this work, we identify the features of Raman spectra of murine primary endothelial cells (EC) isolated from lungs, heart, liver, brain, kidney and aorta of normal mice, as well as from heart, lung and liver in a murine model of heart failure (HF) in Tgaq*44 mice. Primary cells were measured in suspension immediately after their isolation. Raman images showed that isolated primary EC were elliptical or circular, and did not show organ-specific spectral features for any of the studied organ, i.e. lungs, heart, liver, brain, kidney and aorta. Principal Component Analysis pairwise analysis of primary endothelial cells from FVB mice and Tgaq*44 mice revealed an increased protein content in EC isolated from the heart and increased lipid content in EC isolated from the lung in Tgaq*44 mice. No significant differences were found in the EC isolated from the liver using the same chemometric procedure. To our knowledge, this is the first report in which Raman spectroscopy has been used to characterize the biochemical phenotype of primary murine EC with developing HF. This pilot study shows that Raman-based analysis of freshly isolated primary EC did not revealed organ-specific features, however disease-associated changes were found in the coronary and pulmonary EC in the early stage of heart failure in Tgaq*44 mice.

【 授权许可】

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