Niacin bound chromium treatment induces myocardial Glut-4 translocation and caveolar interaction via Akt, AMPK and eNOS phosphorylation in streptozotocin induced diabetic rats after ischemia-reperfusion injury

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1792

Article

Penumathsa, Suresh Varma¹  Thirunavukkarasu, Mahesh¹  Samuel, Samson Mathews¹  Zhan, Lijun¹  Maulik, Gautam²  Bagchi, Manashi³  Bagchi, Debasis³  Maulik, Nilanjana¹ 

[1] Univ Connecticut, Ctr Hlth, Dept Surg, Mol Cardiol & Angiogenesis Lab, Farmington, CT 06030 USA

[2] Harvard Univ, Sch Med, Dept Thorac Surg, Boston, MA USA

[3] InterHlth Res Ctr, Benicia, CA USA

关键词: Chromium;    Diabetes;    eNOS;    Heart;    Glut-4;    Ischemia reperfusion;   

DOI  :  10.1016/j.bbadis.2008.10.018

来源: Elsevier

【 摘 要 】

Diabetes, one of the major risk factors of metabolic syndrome culminates in the development of Ischemic Heart Disease (IHD). Refined diets that lack micronutrients, mainly trivalent chromium (Cr3+) have been identified as the contributor in the rising incidence of diabetes. We investigated the effect of niacin-bound chromium (NBC) during ischemia/reperfusion (IR) injury in streptozotocin induced diabetic rats. Rats were randomized into: Control (Con); Diabetic (Dia) and Diabetic rats fed with NBC (Dia+NBC). After 30 days of treatment, the isolated hearts were subjected to 30 min of global ischemia followed by 2 h of reperfusion. NBC treatment demonstrated significant increase in left ventricular functions and significant reduction in infarct size and cardiomyocyte apoptosis in Dia+NBC compared with Dia. Increased Glut-4 translocation to the lipid raft fractions was also observed in Dia+NBC compared to Dia. Reduced Cav-1 and increased Cav-3 expression along with phosphorylation of Akt, eNOS and AMPK might have resulted in increased Glut-4 translocation in Dia+NBC. Our results indicate that the cardioprotective effect of NBC is mediated by increased activation of AMPK, Akt and eNOS resulting in increased translocation of Glut-4 to the caveolar raft fractions thereby alleviating the effects of IR injury in the diabetic myocardium. (C) 2008 Elsevier B.V. All rights reserved.

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