Cloning of the SmSPO-1 gene preferentially expressed in sporocyst during the life cycle of the parasitic helminth Schistosoma mansoni

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1453

Article

Ram, D ; Lantner, F ; Ziv, E ; Lardans, V ; Schechter, I

关键词: stage-specific gene;    cDNA sequence;    gene sequence;    parasite life cycle;    sporocyst;    (Schistosoma mansoni);   

DOI  :  10.1016/S0925-4439(99)00012-5

来源: Elsevier

【 摘 要 】

Schistosomes are parasitic helminths with a complex life cycle in human and snail hosts. They express stage-specific genes that conceivably determine distinct properties of the parasite at different developmental stages. Here we report the stage-specific gene SmSPO-1, which is preferentially expressed in sporocysts residing in the snail host. The cDNA and the gene were cloned and sequenced. The cDNA. from cap site to the poly(A) addition site, is 498 bp long. It encodes a protein of 117 amino acids with a hydrophobic signal peptide of Is residues, indicating that SmSPO-1 is a secreted or a membranal protein. In the gene the cDNA is split into four exons spread over 2.1 kb of chromosomal DNA. (C) 1999 Elsevier Science B.V. All rights reserved.

【 授权许可】

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