PROTEIN-KINASE ACTIVITIES IN LEISHMANIA-AETHIOPICA - CONTROL BY GROWTH, TRANSFORMATION AND INHIBITORS

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1270

Article

ASSEFA, D ; WORKU, Y ; SKOGLUND, G

关键词: PROTEIN KINASE;    ENZYME ACTIVITY;    (L-AETHIOPICA);   

DOI  :  10.1016/0925-4439(94)00080-A

来源: Elsevier

【 摘 要 】

Promastigotes of L. aethiopica express an ectokinase activity preferring histone V-S as substrate. A soluble kinase activity utilizing protamine and histone V-S, as well as a particulate fraction associated kinase activity preferring protamine are also expressed. The soluble histone kinase activity, but not the ectokinase, was expressed at a higher level in cells from late phases of growth, as compared to early log phase cultures. Transformation of L. aethiopica to an amastigote-like stage, resulted in almost complete loss of the kinase activities, with retained viability of the cells. Formycin-ATP only weakly inhibited the kinases while effectively inhibiting cell growth and thymidine incorporation. Staurosporin efficiently blocked the kinase activities and cell growth without affecting thymidine incorporation.

【 授权许可】

Free