Generation of rat eosinophils by recombinant rat interleukin-5 in vitro and in vivo

BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR BASIS OF DISEASE

卷:1501

Article

Ishihara, K ; Satoh, I ; Mue, S ; Ohuchi, K

关键词: interleukin-5;    eosinophil;    bone marrow cell;    eosinophil differentiation factor;    major basic protein;   

DOI  :  10.1016/S0925-4439(00)00002-8

来源: Elsevier

【 摘 要 】

The addition of recombinant rat interleukin-5 (IL-5), which was purified from the hemolymph of silkworm Bombyx mori larvae infected with IL-5-expressing recombinant virus, to cultures of rat bone marrow cells resulted in an increase in the number of Luxol-fast-blue staining eosinophils in a time- and concentration-dependent manner. After 6 days culture with 100 pM recombinant rat IL-5, more than 90% of the bone marrow cells were eosinophil. The contents of major basic protein (MBP) in the bone marrow cells determined by Western blot analysis using a polyclonal antibody to rat MBP were also increased by recombinant rat IL-5 (100 pM). Furthermore,, intravenous injections of recombinant rat IL-5 twice a day for six consecutive days increased the population of eosinophils in peripheral blood cells and in bone marrow cells. These findings indicate that rat IL-5 induces terminal differentiation and proliferation of progenitor cells to mature eosinophils in rats. (C) 2000 Elsevier Science B.V. All rights reserved.

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