Aggregation ability of three phylogenetically distant anammox bacterial species

WATER RESEARCH

卷:143

Article

Ali, Muhammad^1,2  Shaw, Dario Rangel¹  Zhang, Lei²  Haroon, Mohamed Fauzi³  Narita, Yuko²  Emwas, Abdul-Hamid⁴  Saikaly, Pascal E.¹  Okabe, Satoshi² 

[1] King Abdullah Univ Sci & Technol, Biol & Environm Sci & Engn Div, Water Desalinat & Reuse Ctr, Thuwal 239556900, Saudi Arabia

[2] Hokkaido Univ, Div Environm Engn, Fac Engn, North 13,West 8, Sapporo, Hokkaido 0608628, Japan

[3] Harvard Univ, Dept Organism & Evolutionary Biol, Cambridge, MA 02138 USA

[4] King Abdullah Univ Sci & Technol, Core Labs, Thuwal 239556900, Saudi Arabia

关键词: Anammox bacteria;    Aggregation ability;    Cell surface physicochemical properties;    Extracellular polymeric substances;   

DOI  :  10.1016/j.watres.2018.06.007

来源: Elsevier

【 摘 要 】

Anaerobic ammonium-oxidizing (anammox) bacteria are well known for their aggregation ability. However, very little is known about cell surface physicochemical properties of anammox bacteria and thus their aggregation abilities have not been quantitatively evaluated yet. Here, we investigated the aggregation abilities of three different anammox bacterial species: Candidatus Brocadia sinica, Ca. Jettenia caeni and Ca. Brocadia sapporoensis. Planktonic free-living enrichment cultures of these three anammox species were harvested from the membrane bioreactors (MBRs). The physicochemical properties (e.g., contact angle, zeta potential, and surface thermodynamics) were analyzed for these anammox bacterial species and used in the extended DLVO theory to understand the force-distance relationship. In addition, their extracellular polymeric substances (EPSs) were characterized by X-ray photoelectron spectroscopy and nuclear magnetic resonance. The results revealed that the Ca. B. sinica cells have the most hydrophobic surface and less hydrophilic functional groups in EPS than other anammox strains, suggesting better aggregation capability. Furthermore, aggregate formation and anammox bacterial populations were monitored when planktonic free-living cells were cultured in up-flow column reactors under the same conditions. Rapid development of microbial aggregates was observed with the anammox bacterial population shifts to a dominance of Ca. B. sinica in all three reactors. The dominance of Ca. B. sinica could be explained by its better aggregation ability and the superior growth kinetic properties (higher growth rate and affinity to nitrite). The superior aggregation ability of Ca. B. sinica indicates significant advantages (efficient and rapid start-up of anammox reactors due to better biomass retention as granules and consequently stable performance) in wastewater treatment application. (C) 2018 Published by Elsevier Ltd.

【 授权许可】

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