Metagenomic profiling of ammonia- and methane-oxidizing microorganisms in two sequential rapid sand filters

WATER RESEARCH

卷:185

Article

Poghosyan, Lianna¹  Koch, Hanna¹  Frank, Jeroen¹  van Kessel, Maartje A. H. J.¹  Cremers, Geert¹  van Alen, Theo¹  Jetten, Mike S. M.¹  Op den Camp, Huub J. M.¹  Lucker, Sebastian¹ 

[1] Radboud Univ Nijmegen, Dept Microbiol, Heyendaalseweg 135, NL-6525 AJ Nijmegen, Netherlands

关键词: Sand filtration;    Nitrification;    Comammox;    Nitrospira;    Methanotrophic bacteria;    Metagenomics;   

DOI  :  10.1016/j.watres.2020.116288

来源: Elsevier

【 摘 要 】

Elevated concentrations of ammonium and methane in groundwater are often associated with microbiological, chemical and sanitary problems during drinking water production and distribution. To avoid their accumulation, raw water in the Netherlands and many other countries is purified by sand filtration. These drinking water filtration systems select for microbial communities that mediate the biodegradation of organic and inorganic compounds. In this study, the top layers and wall biofilm of a Dutch drinking water treatment plant (DWTP) were sampled from the filtration units of the plant over three years. We used high-throughput sequencing in combination with differential coverage and sequence composition-based binning to recover 56 near-complete metagenome-assembled genomes (MAGs) with an estimated completion of >= 70% and with >= 10% redundancy. These MAGs were used to characterize the microbial communities involved in the conversion of ammonia and methane. The methanotrophic microbial communities colonizing the wall biofilm (WB) and the granular material of the primary rapid sand filter (P-RSF) were dominated by members of the Methylococcaceae and Methylophilaceae. The abundance of these bacteria drastically decreased in the secondary rapid sand filter (S-RSF) samples. In all samples, complete ammonia-oxidizing (comammox) Nitrospira were the most abundant nitrifying guild. Clade A comammox Nitrospira dominated the P-RSF, while clade B was most abundant in WB and S-RSF, where ammonium concentrations were much lower. In conclusion, the knowledge obtained in this study contributes to understanding the role of microorganisms in the removal of carbon and nitrogen compounds during drinking water production. We furthermore found that drinking water treatment plants represent valuable model systems to study microbial community function and interaction. (c) 2020 The Author(s). Published by Elsevier Ltd.

【 授权许可】

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