Polyphosphate-accumulating organisms in full-scale tropical wastewater treatment plants use diverse carbon sources

WATER RESEARCH

卷:149

Article

Qiu, Guanglei^1,6  Zuniga-Montanez, Rogelio^1,2  Law, Yingyu¹  Thi, Sara Swa¹  Thi Quynh Ngoc Nguyen¹  Eganathan, Kaliyamoorthy³  Liu, Xianghui¹  Nielsen, Per H.^1,4  Williams, Rohan B. H.³  Wuertz, Stefan^1,2,5 

[1] Nanyang Technol Univ, Singapore Ctr Environm Life Sci Engn, Singapore 637551, Singapore

[2] Univ Calif Davis, Dept Civil & Environm Engn, One Shields Ave, Davis, CA 95616 USA

[3] Natl Univ Singapore, Singapore Ctr Environm Life Sci Engn, Singapore 119077, Singapore

[4] Aalborg Univ, Dept Chem & Biosci, Ctr Microbial Commun, DK-9220 Aalborg, Denmark

[5] Nanyang Technol Univ, Sch Civil & Environm Engn, Singapore 639798, Singapore

[6] South China Univ Technol, Sch Environm & Energy, Guangzhou 510006, Guangdong, Peoples R China

关键词: Enhanced biological phosphorus removal (EBPR);    High temperature;    Polyphosphate-accumulating organisms (PAO);    Candidatus Accumulibacter;    Tetrasphaera;    Carbon source;    Volatile fatty acids (VFAs);    Amino acids;    Sugars;    Alcohols;   

DOI  :  10.1016/j.watres.2018.11.011

来源: Elsevier

【 摘 要 】

Enhanced biological phosphorus removal (EBPR) is considered challenging in the tropics, based on a great number of laboratory-based studies showing that the polyphosphate-accumulating organism (PAO) Candidatus Accumulibacter does not compete well with glycogen accumulating organisms (GAOs) at temperatures above 25 degrees C. Yet limited information is available on the PAO community and the metabolic capabilities in full-scale EBPR systems operating at high temperature. We studied the composition of the key functional PAO communities in three full-scale wastewater treatment plants (WWTPs) with high in situ EBPR activity in Singapore, their EBPR-associated carbon usage characteristics, and the relationship between carbon usage and community composition. Each plant had a signature community composed of diverse putative PAOs with multiple operational taxonomic units (OTUs) affiliated to Ca. Accumulibacter, Tetrasphaera spp., Dechloromonas and Ca. Obscuribacter. Despite the differences in community composition, ex-situ anaerobic phosphorus (P)-release tests with 24 organic compounds from five categories (including four sugars, three alcohols, three volatile fatty acids (VFAs), eight amino acids and six other carboxylic acids) showed that a wide range of organic compounds could potentially contribute to EBPR. VFAs induced the highest P release (12.0-18.2 mg P/g MLSS for acetate with a P release-to-carbon uptake (P:C) ratio of 0.35-0.66 mol P/mol C, 9.4-18.5 mg P/g MLSS for propionate with a P:C ratio of 0.38-0.60, and 9.5-17.3 mg P/g MLSS for n-butyrate), followed by some carboxylic acids (10.1-18.1 mg P/g MLSS for pyruvate, 4.5-11.7 mg P/g MLSS for lactate and 3.7-12.4 mg P/g MLSS for fumarate) and amino acids (3.66-7.33 mg P/g MLSS for glutamate with a P:C ratio of 0.16-0.43 mol P/mol C, and 4.01-7.37 mg P/g MLSS for aspartate with a P:C ratio of 0.17-0.48 mol P/mol C). P -release profiles (induced by different carbon sources) correlated closely with PAO community composition. High micro-diversity was observed within the Ca. Accumulibacter lineage, which represented the most abundant PAOs. The total population of Ca. Accumulibacter taxa was highly correlated with P-release induced by VFAs, highlighting the latter's importance in tropical EBPR systems. There was a strong link between the relative abundance of individual Ca. Accumulibacter OTUs and the extent of P release induced by distinct carbon sources (e.g., OTU 81 and amino acids, and OTU 246 and ethanol), suggesting niche differentiation among Ca. Accumulibacter taxa. A diverse PAO community and the ability to use numerous organic compounds are considered key factors for stable EBPR in full-scale plants at elevated temperatures. (C) 2018 Elsevier Ltd. All rights reserved.

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