Germicidal glowsticks: Side-emitting optical fibers inhibit Pseudomonas aeruginosa and Escherichia coli on surfaces

WATER RESEARCH

卷:184

Article

Lanzarini-Lopes, Mariana¹  Zhao, Zhe¹  Perreault, Francois¹  Garcia-Segura, Sergi¹  Westerhoff, Paul¹ 

[1] Arizona State Univ, Sch Sustainable Engn & Built Environm, Nanosyst Engn Res Ctr Nanotechnol Enabled Water T, Tempe, AZ 85287 USA

关键词: Nanotechnology;    Surface disinfection;    Pathogen;    Ultraviolet radiation;    LED;    Biofilm;   

DOI  :  10.1016/j.watres.2020.116191

来源: Elsevier

【 摘 要 】

This paper investigates using UV-C side-emitting optical fibers (SEOFs) to prevent growth of pathogenic bacteria (Pseudomonas aeruginosa and Escherichia coli) on nutrient-rich surfaces. Attaching a SEOF to a single 265 nm light emitting diode (LED) increases irradiation area by >1000x and provides continuous low-irradiance of UV-C light to a large surface area. A zone-of-inhibition protocol was developed to quantify bacterial growth prevention on an agar plate around one SEOF. The inhibition zone increased linearly with irradiance time until achieving a maximum inhibition zone of 2.5 to 3 cm, which received similar to 4.3 mJ/cm(2) of 265 nm light in 2 hours. The surviving lawn edge bacterial colonies did not develop UV resistance after two generations of exposure. The agar plate remained bio-available after UV exposure, and bacteria could be grown on pre-illuminated area in the absence of UV-C light. Whereas we previously demonstrated SEOFs can inactivate planktonic bacteria, herein we show the ability of SEOFs to prevent bacteria growth on surfaces. This is the first step towards developing technologies with multiple SEOFs to inhibit biofilm growth on surfaces, which is a ubiquitous challenge across multiple applications from membrane surfaces to surfaces in pipes or water storage systems. (C) 2020 Elsevier Ltd. All rights reserved.

【 授权许可】

Free