Microbial communities from different types of natural wastewater treatment systems: Vertical and horizontal flow constructed wetlands and biofilters

WATER RESEARCH

卷:55

Article

Adrados, B.^1,2  Sanchez, O.⁴  Arias, C. A.³  Becares, E.⁵  Garrido, L.⁴  Mas, J.⁴  Brix, H.³  Morato, J.^1,2 

[1] Univ Politecn Cataluna, Opt & Optometry Dept, Hlth & Environm Microbiol Lab, Terrassa 08222, Spain

[2] Univ Politecn Cataluna, AQUASOST UNESCO Chair Sustainabil, Terrassa 08222, Spain

[3] Aarhus Univ, Dept Biosci, DK-8000 Aarhus C, Denmark

[4] Univ Autonoma Barcelona, Dept Genet & Microbiol, Bellaterra 08193, Spain

[5] Univ Leon, Fac Ciencias Biol & Ambientales, E-24071 Leon, Spain

关键词: Microbial community;    PCR-DGGE;    Domestic wastewater;    Constructed wetlands;    Biofilters;   

DOI  :  10.1016/j.watres.2014.02.011

来源: Elsevier

【 摘 要 】

The prokaryotic microbial communities (Bacteria and Archaea) of three different systems operating in Denmark for the treatment of domestic wastewater (horizontal flow constructed wetlands (HFCW), vertical flow constructed wetlands (VFCW) and biofilters (BF)) was analysed using endpoint PCR followed by Denaturing Gradient Gel Electrophoresis (DGGE). Further sequencing of the most representative bacterial bands revealed that diverse and distinct bacterial communities were found in each system unit, being gamma-Proteobacteria and Bacteroidetes present mainly in all of them, while Firmicutes was observed in HFCW and BF. Members of the Actinobacteria group, although found in HFCW and VFCW, seemed to be more abundant in BF units. Finally, some representatives of alpha, beta and delta-Proteobacteria, Acidobacteria and Chloroflexi were also retrieved from some samples. On the other hand, a lower archaeal diversity was found in comparison with the bacterial population. Cluster analysis of the DGGE bacterial band patterns showed that community structure was related to the design of the treatment system and the organic matter load, while no clear relation was established between the microbial assemblage and the wastewater influent. (C) 2014 Elsevier Ltd. All rights reserved.

【 授权许可】

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