High-resolution within-sewer SARS-CoV-2 surveillance facilitates informed intervention

WATER RESEARCH

卷:204

Article

Reeves, Katelyn^1,2  Liebig, Jennifer³  Feula, Antonio³  Saldi, Tassa³  Lasda, Erika^1,2  Johnson, William¹  Lilienfeld, Jacob^1,2  Maggi, Juniper^1,2  Pulley, Kevin^1,2  Wilkerson, Paul J.^1,2  Real, Breanna^1,2  Zak, Gordon^1,2  Davis, Jack³  Fink, Morgan³  Gonzales, Patrick³  Hager, Cole³  Ozeroff, Christopher³  Tat, Kimngan³  Alkire, Michaela^1,2  Butler, Claire^1,2  Coe, Elle^1,2  Darby, Jessica^1,2  Freeman, Nicholas^1,2  Heuer, Heidi^1,2  Jones, Jeffery R.^1,2  Karr, Madeline^1,2  Key, Sara^1,2  Maxwell, Kiersten^1,2  Nelson, Lauren^1,2  Saldana, Emily^1,2  Shea, Rachel^1,2  Salveson, Lewis^1,2  Tomlinson, Kate^1,2  Vargas-Barriga, Jorge^1,2  Vigil, Bailey^1,2  Brisson, Gloria⁴  Parker, Roy³  Leinwand, Leslie A.^3,5  Bjorkman, Kristen³  Mansfeldt, Cresten^1,2 

[1] Univ Colorado, Dept Civil Environm & Architectural Engn, 1111 Engn Dr, Boulder, CO 80309 USA

[2] Univ Colorado, Environm Engn Program, 4001 Discovery Dr, Boulder, CO 80303 USA

[3] Univ Colorado, BioFrontiers Inst, 3415 Colorado Ave, Boulder, CO 80303 USA

[4] Univ Colorado, Med Serv, 1900 Wardenburg Dr, Boulder, CO 80309 USA

[5] Univ Colorado, Dept Mol Cellular & Dev Biol, 1945 Colorado Ave, Boulder, CO 80309 USA

关键词: SARS-CoV-2;    COVID-19;    Wastewater surveillance;    Wastewater-based epidemiology;    RT-qPCR;    Composite sampler;    Building-scale monitoring;   

DOI  :  10.1016/j.watres.2021.117613

来源: Elsevier

【 摘 要 】

To assist in the COVID-19 public health guidance on a college campus, daily composite wastewater samples were withdrawn at 20 manhole locations across the University of Colorado Boulder campus. Low-cost autosamplers were fabricated in-house to enable an economical approach to this distributed study. These sample stations operated from August 25th until November 23rd during the fall 2020 semester, with 1512 samples collected. The concentration of SARS-CoV-2 in each sample was quantified through two comparative reverse transcription quantitative polymerase chain reactions (RT-qPCRs). These methods were distinct in the utilization of technical replicates and normalization to an endogenous control. (1) Higher temporal resolution compensates for supply chain or other constraints that prevent technical or biological replicates. (2) The data normalized by an endogenous control agreed with the raw concentration data, minimizing the utility of normalization. The raw wastewater concentration values reflected SARS-CoV-2 prevalence on campus as detected by clinical services. Overall, combining the low-cost composite sampler with a method that quantifies the SARS-CoV-2 signal within six hours enabled actionable and time-responsive data delivered to key stakeholders. With daily reporting of the findings, wastewater surveillance assisted in decision making during critical phases of the pandemic on campus, from detecting individual cases within populations ranging from 109 to 2048 individuals to monitoring the success of on-campus interventions.

【 授权许可】

Free