Inhibition of alkaline flocculation by algal organic matter for Chlorella vulgaris

WATER RESEARCH

卷:88

Article

Vandamme, Dries¹  Beuckels, Annelies¹  Vadelius, Eric⁶  Depraetere, Orily¹  Noppe, Wim²  Dutta, Abhishek³  Foubert, Imogen^4,5  Laurens, Lieve⁶  Muylaert, Koenraad¹ 

[1] KU Leuven Kulak, Lab Aquat Biol, B-8500 Kortrijk, Belgium

[2] KU Leuven Kulak, IRF Life Siences, B-8500 Kortrijk, Belgium

[3] Katholieke Univ Leuven, Fac Ind Ingenieurswetenschappen, B-3000 Leuven, Belgium

[4] KU Leuven Kulak, Res Unit Food & Lipids, Dept Mol & Microbial Syst Kulak, B-8500 Kortrijk, Belgium

[5] Katholieke Univ Leuven, Leuven Food Sci & Nutr Res Ctr LFoRCe, B-3001 Heverlee, Belgium

[6] Natl Renewable Energy Lab, Natl Bioenergy Ctr, Golden, CO 80401 USA

关键词: Extracellular polymeric substances;    Dissolved organic matter;    Coagulation;    Harvesting;    Microalgae;    Biofuels;   

DOI  :  10.1016/j.watres.2015.10.032

来源: Elsevier

【 摘 要 】

Alkaline flocculation is a promising strategy for the concentration of microalgae for bulk biomass production. However, previous studies have shown that biological changes during the cultivation negatively affect flocculation efficiency. The influence of changes in cell properties and in the quality and composition of algal organic matter (AOM) were studied using Chlorella vulgaris as a model species. In batch cultivation, flocculation was increasingly inhibited over time and mainly influenced by changes in medium composition, rather than biological changes at the cell surface. Total carbohydrate content of the organic matter fraction sized bigger than 3 kDa increased over time and this fraction was shown to be mainly responsible for the inhibition of alkaline flocculation. The monosaccharide identification of this fraction mainly showed the presence of neutral and anionic monosaccharides. The addition of 30 -50 mg L-1 alginic acid, as a model for anionic carbohydrate polymers containing uronic acids, resulted in a complete inhibition of flocculation. These results suggest that inhibition of alkaline flocculation was caused by interaction of anionic polysaccharides leading to an increased flocculant demand over time. (C) 2015 Elsevier Ltd. All rights reserved.

【 授权许可】

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