Temporal dynamics of norovirus determined through monitoring of municipal wastewater by pyrosequencing and virological surveillance of gastroenteritis cases

WATER RESEARCH

卷:92

Article

Kazama, Shinobu¹  Masago, Yoshifumi^1,2  Tohma, Kentaro³  Souma, Nao³  Imagawa, Toshifumi³  Suzuki, Akira⁴  Liu, Xiaofang³  Saito, Mayuko³  Oshitani, Hitoshi³  Omura, Tatsuo¹ 

[1] Tohoku Univ, New Ind Creat Hatchery Ctr, Sendai, Miyagi 9808479, Japan

[2] United Nations Univ, Inst Adv Study Sustainabil, Shibuya Ku, Tokyo 1508925, Japan

[3] Tohoku Univ, Tohoku Grad Sch Med, Dept Virol, Sendai, Miyagi 9808575, Japan

[4] Sendai Med Ctr, Virus Res Ctr, Div Clin Res, Sendai, Miyagi 9838520, Japan

关键词: Norovirus;    Wastewater;    Pyrosequencing;    Virological surveillance;   

DOI  :  10.1016/j.watres.2015.10.024

来源: Elsevier

【 摘 要 】

Norovirus is a leading etiological agent of viral gastroenteritis. Because of relatively mild disease symptoms and frequent asymptomatic infections, information on the ecology of this virus is limited. Our objective was to examine the genetic diversity of norovirus circulating in the human population by means of genotyping the virus in municipal wastewater. We investigated norovirus genogroups I and II (GI and GII) in municipal wastewater in Japan by pyrosequencing and quantitative PCR (qPCR) from November 2012 to March 2013. Virological surveillance for gastroenteritis cases was concurrently conducted in the same area. A total of fourteen distinct genotypes in total (GI.1, 3, 4, 6, 7, GII.2, 4, 5, 6, 7,12,13, 14, and 17), with up to eight genotypes detected per sample, were observed in wastewater using pyrosequencing; only four genotypes (GI.6, GII.4, 5, and 14) were obtained from clinical samples. Seventy-eight percent of norovirus-positive stool samples contained GII.4, but this genotype was not dominant in wastewater. The norovirus GII.4 Sydney 2012 variant, which appeared and spread during our study period, was detected in both the wastewater and clinical samples. These results suggest that an environmental approach using pyrosequencing yields a more detailed distribution of norovirus genotypes/variants. Thus, wastewater monitoring by pyrosequencing is expected to provide an effective analysis of the distribution of norovirus genotypes causing symptomatic and asymptomatic infections in human populations. (C) 2015 Elsevier Ltd. All rights reserved.

【 授权许可】

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