Next generation sequencing approaches to evaluate water and wastewater quality

WATER RESEARCH

卷:194

Review

Garner, Emily¹  Davis, Benjamin C.²  Milligan, Erin²  Blair, Matthew Forrest²  Keenum, Ishi²  Maile-Moskowitz, Ayella²  Pan, Jin²  Gnegy, Mariah²  Liguori, Krista²  Gupta, Suraj³  Prussin, Aaron J., II²  Marr, Linsey C.²  Heath, Lenwood S.⁴  Vikesland, Peter J.²  Zhang, Liqing⁴  Pruden, Amy² 

[1] West Virginia Univ, Wadsworth Dept Civil & Environm Engn, 1306 Evansdale Dr, Morgantown, WV 26505 USA

[2] Virginia Tech, Dept Civil & Environm Engn, 1145 Perry St, Blacksburg, VA 24061 USA

[3] Virginia Tech, Interdisciplinary PhD Program Genet Bioinformat &, Blacksburg, VA 24061 USA

[4] Virginia Tech, Dept Comp Sci, 225 Stranger St, Blacksburg, VA 24061 USA

关键词: Next generation sequencing;    Drinking water;    Wastewater;    Water reuse;    Metagenomics;    Metatranscriptomics;   

DOI  :  10.1016/j.watres.2021.116907

来源: Elsevier

【 摘 要 】

The emergence of next generation sequencing (NGS) is revolutionizing the potential to address complex microbiological challenges in the water industry. NGS technologies can provide holistic insight into micro-bial communities and their functional capacities in water and wastewater systems, thus eliminating the need to develop a new assay for each target organism or gene. However, several barriers have hampered wide-scale adoption of NGS by the water industry, including cost, need for specialized expertise and equipment, challenges with data analysis and interpretation, lack of standardized methods, and the rapid pace of development of new technologies. In this critical review, we provide an overview of the current state of the science of NGS technologies as they apply to water, wastewater, and recycled water. In addi-tion, a systematic literature review was conducted in which we identified over 600 peer-reviewed jour-nal articles on this topic and summarized their contributions to six key areas relevant to the water and wastewater fields: taxonomic classification and pathogen detection, functional and catabolic gene charac-terization, antimicrobial resistance (AMR) profiling, bacterial toxicity characterization, Cyanobacteria and harmful algal bloom identification, and virus characterization. For each application, we have presented key trends, noteworthy advancements, and proposed future directions. Finally, key needs to advance NGS technologies for broader application in water and wastewater fields are assessed. (c) 2021 Elsevier Ltd. All rights reserved.

【 授权许可】

Free