Interaction of bacterial communities and indicators of water quality in shoreline sand, sediment, and water of Lake Michigan

WATER RESEARCH

卷:178

Article

Nevers, Meredith B.¹  Byappanahalli, Muruleedhara N.¹  Nakatsu, Cindy H.²  Kinzelman, Julie L.³  Phanikumar, Mantha S.⁴  Shively, Dawn A.⁴  Spoljaric, Ashley M.⁴ 

[1] US Geol Survey, Great Lakes Sci Ctr, 1574 North 300 East, Chesterton, IN 46304 USA

[2] Purdue Univ, Dept Agron, 915 W State St, W Lafayette, IN 47907 USA

[3] City Racine Publ Hlth Dept, 730 Washington Ave, Racine, WI 53403 USA

[4] Michigan State Univ, Dept Civil & Environm Engn, E Lansing, MI 48824 USA

关键词: Targeted metagenomic 16S rRNA gene analysis;    Microbial source tracking;    Indicator bacteria;    Great Lakes;   

DOI  :  10.1016/j.watres.2020.115671

来源: Elsevier

【 摘 要 】

Shoreline sand harbors high concentrations of fecal indicator bacteria (FIB) that may be resuspended into the water column through washing and resuspension. Studies have explored coastal processes that influence this sand-water flux for FIB, but little is known about how microbial markers of contamination or the bacterial community interact in the sand-water interface. In this study, we take a three-tiered approach to explore the relationship between bacteria in sand, sediment, and overlying water at three shoreline sites and two associated rivers along an extended freshwater shoreline. Samples were collected over two years and analyzed for FIB, two microbial source tracking (MST) markers (Catellicoccus marimammalium, Gull2; Bacteroides HF183), and targeted metagenomic 16S rRNA gene analysis. FIB was much higher in sand than in water at all three sites. Gull2 marker was abundant in shoreline sand and water while HF183 marker was mostly present in rivers. Overall bacterial communities were dissimilar between sand/sediment and water, indicating little interaction. Sediment composition was generally unfavorable to bacterial resuspension. Results show that FIB and MST markers were effective estimates of short-term conditions at these locations, and bacterial communities in sand and sediment reflected longer-term conditions. Findings are useful for locating contamination sources and targeting restoration by evaluating scope of shoreline degradation. (C) 2020 Published by Elsevier Ltd.

【 授权许可】

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