Transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms

WATER RESEARCH

卷:141

Article

Falas, Per^1,2  Jewell, Kevin S.³  Hermes, Nina³  Wick, Arne³  Ternes, Thomas A.³  Joss, Adriano¹  Nielsen, Jeppe Lund⁴ 

[1] Swiss Fed Inst Aquat Sci & Technol, Eawag, Uberlandstr 133, CH-8600 Dubendorf, Switzerland

[2] Lund Univ, Dept Chem Engn, Water & Environm Engn, S-22100 Lund, Sweden

[3] Fed Inst Hydrol, Mainzer Tor 1, D-56068 Koblenz, Germany

[4] Aalborg Univ, Dept Chem & Biosci, Ctr Microbial Communities, Fredrik Bajers Vej 7H, DK-9220 Aalborg, Denmark

关键词: Micropollutants;    Transformation;    Biological wastewater treatment;    Moving bed biofilm reactors;    Microautoradiography;   

DOI  :  10.1016/j.watres.2018.03.040

来源: Elsevier

【 摘 要 】

A tiered process was developed to assess the transformation, CO2 formation and uptake of four organic micropollutants by carrier-attached microorganisms from two municipal wastewater treatment plants. At the first tier, primary transformation of ibuprofen, naproxen, diclofenac, and mecoprop by carrier attached microorganisms was shown by the dissipation of the target compounds and the formation of five transformation products using LC-tandem MS. At the second tier, the microbial cleavage of the four organic micropollutants was confirmed with C-14-labeled micropollutants through liquid scintillation counting of the (CO2)-C-14 formed. At the third tier, microautoradiography coupled with fluorescence in situ hybridization (MAR-FISH) was used to screen carrier-attached microorganisms for uptake of the four radiolabeled micropollutants. Results from the MAR-FISH screening indicated that only a small fraction of the microbial community (<= 1 parts per thousand) was involved in the uptake of the radiolabeled micropollutants and that the responsible microorganisms differed between the compounds. At the fourth tier, the microbial community structure of the carrier-attached biofilms was analyzed by 16S rRNA gene amplicon sequencing. The sequencing results showed that the MAR-FISH screening targeted similar to 80% of the microbial community and that several taxonomic families within the FISH-probed populations with MAR-positive signals (i.e. Firmicutes, Gammaproteobacteria, and Deltaproteobacteria) were present in both biofilms. From the broader perspective of organic micropollutant removal in biological wastewater treatment, the MAR-FISH results of this study indicate a high degree of microbial substrate specialization that could explain differences in transformation rates and patterns between micropollutants and microbial communities. (C) 2018 Elsevier Ltd. All rights reserved.

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