Nitrogen transforming community in a horizontal subsurface-flow constructed wetland

WATER RESEARCH

卷:74

Article

Coban, Oksana¹  Kuschk, Peter²  Kappelmeyer, Uwe²  Spott, Oliver³  Martienssen, Marion⁴  Jetten, Mike S. M.⁵  Knoeller, Kay¹ 

[1] UFZ Helmholtz Ctr Environm Res, Dept Catchment Hydrol, D-06120 Halle, Germany

[2] UFZ Helmholtz Ctr Environm Res, Dept Environm Biotechnol, D-04318 Leipzig, Germany

[3] UFZ Helmholtz Ctr Environm Res, Dept Soil Phys, D-06120 Halle, Germany

[4] BTU Cottbus Senftenberg, Dept Biotechnol Water Treatment, Cottbus, Germany

[5] Radboud Univ Nijmegen, Dept Microbiol, Inst Water & Wetland Res, NL-6525 ED Nijmegen, Netherlands

关键词: Nitrification;    Anammox;    Aerobic denitrification;    Abundance;    Activity;    Constructed wetland;   

DOI  :  10.1016/j.watres.2015.02.018

来源: Elsevier

【 摘 要 】

Constructed wetlands are important ecosystems with respect to nitrogen cycling. Here we studied the activity and abundance of nitrogen transforming bacteria as well as the spatial distribution of nitrification, anaerobic ammonium oxidation (anammox), and denitrification processes in a horizontal subsurface-flow constructed wetland. The functional genes of the nitrogen cycle were evenly distributed in a linear way along the flow path with prevalence at the superficial points. The same trend was observed for the nitrification and denitrification turnover rates using isotope labeling techniques. It was also shown that only short-term incubations should be used to measure denitrification turnover rates. Significant nitrate consumption under aerobic conditions diminishes nitrification rates and should therefore be taken into account when estimating nitrification turnover rates. This nitrate consumption was due to aerobic denitrification, the rate of which was comparable to that for anaerobic denitrification. Consequently, denitrification should not be considered as an exclusively anaerobic process. Phylogenetic analysis of hydrazine synthase (hzsA) gene clones indicated the presence of Brocadia and Kuenenia anammox species in the constructed wetland. Although anammox bacteria were detected by molecular methods, anammox activity could not be measured and hence this process appears to be of low importance in nitrogen transformations in these freshwater ecosystems. (C) 2015 Elsevier Ltd. All rights reserved.

【 授权许可】

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