Placement of an Elastic Biodegradable Cardiac Patch on a Subacute Infarcted Heart Leads to Cellularization With Early Developmental Cardiomyocyte Characteristics

JOURNAL OF CARDIAC FAILURE

卷:18

Article

Fujimoto, Kazuro L.^1,2  Tobita, Kimimasa^1,3,4,5  Guan, Jianjun^1,6  Hashizume, Ryotaro^1,2  Takanari, Keisuke^1,2  Alfieri, Christina M.⁷  Yutzey, Katherine E.⁷  Wagner, William R.^1,2,4,5 

[1] Univ Pittsburgh, McGowan Inst Regenerat Med, Pittsburgh, PA 15219 USA

[2] Univ Pittsburgh, Dept Surg, Pittsburgh, PA 15219 USA

[3] Univ Pittsburgh, Dept Dev Biol, Pittsburgh, PA 15219 USA

[4] Univ Pittsburgh, Dept Bioengn, Pittsburgh, PA 15219 USA

[5] Univ Pittsburgh, Dept Chem Engn, Pittsburgh, PA 15219 USA

[6] Ohio State Univ, Dept Mat Sci & Engn, Columbus, OH 43210 USA

[7] Cincinnati Childrens Med Ctr, Div Mol Cardiovasc Biol, Cincinnati, OH USA

关键词: Infarction;    remodeling;    patch implantation;    cellularization;   

DOI  :  10.1016/j.cardfail.2012.05.006

来源: Elsevier

【 摘 要 】

Background: Placement of an elastic biodegradable patch onto a subacute myocardial infarct (MI) provides temporary elastic support that may act to effectively alter adverse left ventricular (LV) remodeling processes. Methods: Two weeks after permanent left coronary ligation in Lewis rats, the infarcted anterior wall was covered with polyester urethane urea (MI + PEUU; n = 15) or expanded polytetratluoroethylene (MI + ePTFE; n = 15) patches, or had no implantation (MI + sham; n = 12). Eight weeks after surgery, cardiac function and histology were assessed. Results: The ventricular wall in the MI + ePTFE and MI + sham groups was composed of fibrous tissue, whereas PEUU implantation induced alpha-smooth muscle actin positive muscle bundles coexpressing sarcomeric alpha-actinin and cardiac-specific troponin-T. This pattern of colocalization was also found in developing embryonic myocardium. Cardiac transcription factors Nkx-2.5 and GATA-4 were strongly expressed in the muscle bundles. In the MI + sham group, end-diastolic LV cavity area (EDA) increased and the percentage of fractional area change (%FAC) decreased. For ePTEE patched animals, both EDA and %FAC decreased. In contrast, with MI + PEUU patching, %FAC increased and EDA was maintained. With dobutamine-stress echocardiography, MI + PEUU patched LVs possessed contractile reserve significantly larger than the MI + sham group. Conclusions: MI + PEUU patch implantation onto subacute infarcted myocardium induced muscle cellularization with characteristics of early developmental cardiomyocytes as well as providing a functional reserve. (J Cardiac Fail 2012:18:585-595)

【 授权许可】

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