Multi-omics analysis reveals interferon-stimulated gene OAS1 as a prognostic and immunological biomarker in pan-cancer

Frontiers in Immunology

Immunology

Yi Liu¹  Hui Feng¹  Fan Niu¹  Yue Du¹  Ruimin Liu¹  Runyu Yang¹  Mengyao Zhang¹  Bingyu Yang¹  Pengcheng He¹  Jiayi Xiao¹ 

[1] Department of Hematology, First Affiliated Hospital of Xi’an Jiaotong University, Xi’an, Shaanxi, China;

关键词: OAS1;    tumor immune microenvironment;    immunotherapeutic resistance;    T-cell dysfunction;    pan-cancer;   

DOI  :  10.3389/fimmu.2023.1249731

 received in 2023-06-29, accepted in 2023-10-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionOAS1(2’-5’-oligoadenylate synthetase 1) is a member of the Interferon-Stimulated Genes which plays an important role in the antiviral process. In recent years, the role of OAS1 in tumors has attracted attention, and it was found to be associated with prognosis in several tumors. However, the mechanism by which OAS1 affects tumors is unclear and pan-cancer study of OAS1 is necessary to better understand its implication in cancers.MethodsThe expression, prognostic value, genetic alteration, alternative splicing events of OAS1 in pan-cancers were analyzed using TCGA, GTEx, HPA, GEPIA and OncoSplicing databases. OAS1 associated immune cell infiltration was evaluated using the ESTIMATE, xCell, CIBERSORT and QUANTISEQ algorithm. Single cell transcriptome data download using TISH database. Finally, the roles of the OAS1 on apoptosis, migration and invasion were investigated in two pancreatic cancer cells.ResultsOur results revealed significant differences in OAS1 expression among various tumors, which had prognostic implications. In addition, we investigated the impact of OAS1 on genomic stability, methylation status, and other factors across different types of cancer, and the effects of these factors on prognosis. Notably, our study also demonstrated that OAS1 overexpression can contribute to CTL dysfunction and macrophage M2 polarization. In addition, cell experiments showed that the knockdown of OAS1 could reduce the invasive ability and increased the apoptosis rate of PAAD cells.DiscussionThese results confirmed that OAS1 could be a prognostic biomarker and therapeutic target for its potential role in CTL dysfunction and macrophage M2 polarization.

【 授权许可】

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Copyright © 2023 Yang, Du, Zhang, Liu, Feng, Liu, Yang, Xiao, He and Niu