Electrokinetic properties of healthy and β-thalassemia erythrocyte membranes under in vitro exposure to static magnetic field

Frontiers in Chemistry

Chemistry

Virjinia Doltchinkova¹  Elitsa Ivanova¹  Blaga Rukova²  Rumin Nikolov³  Chavdar Roumenin⁴  Siya Lozanova⁴ 

[1] Department of Biophysics and Radiobiology, Faculty of Biology, Sofia University “St. Kliment Ohridski”, Sofia, Bulgaria;Department of Medical Genetics, Medical University of Sofia, Sofia, Bulgaria;Faculty of Mechanical Engineering, Technical University, Sofia, Bulgaria;Institute of Robotics “St. Ap. and Gospeller Matthew”, Bulgarian Academy of Sciences, Sofia, Bulgaria;

关键词: static magnetic field;    zeta potential;    erythrocyte membranes;    microelectrophoresis;    beta-thalassemia;    lipid peroxidation;    fluorescence microscopy;   

DOI  :  10.3389/fchem.2023.1197210

 received in 2023-03-30, accepted in 2023-09-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: The current understanding of the biological impacts of a static magnetic field (SMF) is restricted to the direct interactions of the magnetic field with biological membranes. The electrokinetic (zeta) potential is an electrochemical property of erythrocyte surfaces which was negatively charged in physiological media after SMF exposure (0.1‒2.0 T).Methods: The novel data about electrokinetic parameters of the erythrocytes is determined by microelectrophoresis after SMF-exposure in norm and heterozygous β-thalassemia. The methods of light scattering, lipid peroxidation, fluorescence microscopy are used.Results: The electrokinetic potential of erythrocytes in norm is increased after SMF intensities due to enhanced negatively exposed charges on the outer surface of the membrane accompanied by an increase in light scattering where changes in cell morphology are observed. Conversely, a decrease in the zeta potential of β-thalassemia erythrocytes upon SMF-treatment was determined because of the reduction in the surface electrical charge of the membranes, where a significant decrease in light scattering at 1.5 T and 2.0 T was recorded. Exposure to SMF (0.5–2.0 T) was associated with an increase in the malondialdehyde content in erythrocytes. Biophysical studies regarding the influence of SMF on the electrostatic free energy of cells shows an increase in negative values in healthy erythrocytes, which corresponds to the implementation of a spontaneous process. This is also the process in β-thalassemia cells after SMF exposure with lower negative values of free electrostatic energy than erythrocytes in norm.Discussion: The effect of static magnetic field (SMF 0.1–2.0 T) on the electrokinetic and morphological characteristics of erythrocytes in norm and β-thalassemia is determined and correlated with the increase/reduction in surface charge and shrinkage/swelling of the cells, respectively. Lipid peroxidation of healthy and β-thalassemia erythrocytes caused an enhancement of lipid peroxidation because of the higher concentrations of TBARS products in cellular suspension. SMF (0.1‒2.0 T) altered the spontaneous chemical processes with negative values of electrostatic free energy of erythrocytes in norm and β-thalassemia accompanied by a lower FITC-Concanavalin A binding affinity to membrane receptors (SMF 2.0 T). The electrokinetic properties of human erythrocytes in norm and β-thalassemia upon SMF treatment and their interrelationship with the structural-functional state of the membrane were reported. The presented work would have future fundamental applications in biomedicine.

【 授权许可】

Unknown   
Copyright © 2023 Doltchinkova, Lozanova, Rukova, Nikolov, Ivanova and Roumenin.