Combining metal nanoparticles and nanobodies to boost the biomedical imaging in neurodegenerative diseases

Frontiers in Nanotechnology

Nanotechnology

G. Gigli¹  D. Simeone¹  A. Quarta¹  F. Palermo²  A. Sanna³  L. Massimi⁴  I. Viola⁴  I. Bukreeva⁴  E. Quintiero⁴  N. Pieroni⁴  A. Cedola⁴  N. Kerlero de Rosbo⁵  M. Fratini⁵  B. Parodi⁶  A. Bravin⁷  X. Le Guével⁸  L. Sancey⁸ 

[1] CNR-Institute of Nanotechnology, Lecce, Italy;CNR-Institute of Nanotechnology, Lecce, Italy;CNR-Institute of Nanotechnology, Rome, Italy;CNR-Institute of Nanotechnology, Lecce, Italy;CNR-Institute of Nanotechnology, Rome, Italy;INSERM U 1209, CNRS UMR 5309, Institute for Advanced Biosciences, University Grenoble Alpes, Grenoble, France;CNR-Institute of Nanotechnology, Rome, Italy;CNR-Institute of Nanotechnology, Rome, Italy;Laboratory of Neurophysics and Neuroimaging (NaN), IRCCS Fondazione Santa Lucia, Rome, Italy;Department of Neurosciences, Rehabilitation, Ophthalmology, and Maternal-Fetal Medicine (DINOGMI), University of Genoa, Genoa, Italy;Department of Physics “G Occhialini”, University Milano Bicocca, Milano, Italy;Department of Physics, Università della Calabria, Cosenza, Italy;CNR-Nanotec, Rende, Italy;INSERM U 1209, CNRS UMR 5309, Institute for Advanced Biosciences, University Grenoble Alpes, Grenoble, France;

关键词: metal nanoparticles;    nanobodies;    brain;    imaging;    neurodegenenerative diseases;   

DOI  :  10.3389/fnano.2023.1236810

 received in 2023-06-08, accepted in 2023-08-10,  发布年份 2023

来源: Frontiers

【 摘 要 】

Introduction: In the study of neurodegenerative diseases, the possibility to follow the fate of specific cells or molecules within the whole body would be a milestone to better understand the complex evolution of disease mechanisms and to monitor the effects of therapies. The techniques available today do not allow the visualization of disease-relevant cells within the whole tridimensional biological context at high spatial resolution.Methods: Here we show the results from the first validation steps of a novel approach: by combining the conjugate nanobodies anti-glial fibrillary acidic protein (GFAP) and metal-nanoparticles (i.e. 2 nm gold NP) with X-ray phase contrast tomography (XPCT) we would be able to obtain a tridimensional visualization and identification of cells of interest together with the surrounding tissue and the vascular and neuronal networks.Results: By exploiting the X-ray attenuation properties of metal nanoparticles and the specific targeting capabilities of nanobodies, we could give XPCT the specificity it presently lacks, making it no longer a pure morphological but a molecular and targeted imaging technique. In our case, we synthesized and characterized Gold-NP/GFAP nanobody to target the astrocytes of mouse brain.Discussion: The results of the first tests presented in this paper have provided us with information on the feasibility of the approach, encouraging us to carry out further experiments in order to achieve the ultimate goal of setting up this new imaging technique.

【 授权许可】

Unknown   
Copyright © 2023 Sanna, Quarta, Pieroni, Parodi, Palermo, Bukreeva, Fratini, Massimi, Simeone, Le Guével, Bravin, Quintiero, Viola, Gigli, Kerlero de Rosbo, Sancey and Cedola.