Embryogenesis of an aquaculture fish (Dicentrarchus labrax) under simulated altered gravity

Frontiers in Space Technologies

Space Technologies

Gilbert Dutto¹  Raphaelle Paounov¹  Cyrille Przybyla¹  Emmanuel Mansuy¹  Stephane Lallement¹  Benjamin Geffroy¹  Amelys Debiol¹  Julie Bonnefoy²  Jean Pol Frippiat²  Florence Coste² 

[1] MARBEC, Université de Montpellier, CNRS, IFREMER, IRD, France;Université de Lorraine, SIMPA, Nancy, France;

关键词: Complement component 3;    European seabass;    glucocorticoid receptors;    heat shock protein 90;    hypergravity;    interleukin-1 beta;    microgravity;    space aquaculture;   

DOI  :  10.3389/frspt.2023.1240251

 received in 2023-06-14, accepted in 2023-09-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Food production and balanced nutrition will be a key challenge for residents of a future base on the Moon or Mars. As a complement to photosynthetic organisms, space aquaculture could provide the range of amino acids required to maintain health. This would rely on shipping fertilized aquaculture fish eggs to the Moon. To determine the feasibility of this, this study sought to test the influence of the conditions of a lunar mission—such as hypergravity during rocket launch and microgravity during the journey—on fish embryos and young larvae. To analyze the potential effects of these gravity changes on the early developmental stages of fish, we conducted two experiments to expose them to: i) 10 min of simulated hypergravity at 5 g (launch duration) and ii) 39 h of simulated microgravity using a random positioning machine. Both experiments used European sea bass (Dicentrarchus labrax) as a model. We analyzed egg oxygen consumption and hatching rates, as well as the expression of genes related to stress and immunity. The results indicated that neither of these altered gravity conditions affected the hatching rate. Simulated microgravity did not impact fish embryo oxygen consumption and appeared to induce faster embryonic development, as the eggs hatched earlier than expected. Levels of glucocorticoid receptors (GR1 and GR2) and heat shock proteins (Hsp90) were not impacted. Only the levels of complement component protein 3 (C3) were significantly higher in simulated microgravity, while interleukin-1β (IL-1β) transcripts were significantly lower in the hypergravity group, compared to controls. This shows that proteins involved in the innate immune system are expressed under altered gravity. Although further experiments are needed, these results suggest that the European sea bass is a promising candidate for space aquaculture.

【 授权许可】

Unknown   
Copyright © 2023 Przybyla, Bonnefoy, Paounov, Debiol, Dutto, Mansuy, Lallement, Coste, Geffroy and Frippiat.