Altered expression of circular RNA in patients with cervical artery dissection

Frontiers in Neurology

Neurology

Wenru Tan¹  Jie Li¹  Yudi Li¹  Yifan Wang¹  Li Ling¹  Jianyi Mai¹  Siqi Yang¹  Yajie Liu¹  Zhaofei Dong² 

[1] Department of Neurology, Shenzhen Hospital, Southern Medical University, Shenzhen, Guangdong, China;Departments of Neurology, The Eighth Affiliated Hospital, Sun Yat-sen University, Shenzhen, Guangdong, China;

关键词: cervical artery dissection;    circRNA;    ischemic stroke;    high-throughput sequencing;    biomark;   

DOI  :  10.3389/fneur.2023.1228400

 received in 2023-05-27, accepted in 2023-09-18,  发布年份 2023

来源: Frontiers

【 摘 要 】

Cervical artery dissection (CeAD), a special cerebrovascular disease and the main cause of stroke in young people, can present with ischemic stroke, headache, subarachnoid hemorrhage, and other symptoms, increasing the possibility of misdiagnosis. As a special class of non-coding RNAs, circRNAs are commonly found in organisms and can play regulatory roles in transcription and post-transcription processes, affecting gene expression.CircRNAs have reported to be associated with neurological diseases; however, their role in CeAD has not been discerned. In this study, we aimed to elucidate the pathophysiological changes in patients with CeAD and identify biomarkers. Peripheral blood mononuclear cells from patients with CeAD and healthy controls were sequenced using high-throughput sequencing. We detected 460 differently expressed circRNAs in patients with CeAD (p < 0.5, fold difference ≥ 2), of which 240 were upregulated and 220 were downregulated. Four circRNAs showed significant differences in expression, which were validated using qRT-PCR. These results suggested that three circRNAs were consistent with high-throughput sequencing results. Bioinformatics analysis demonstrated that these differentially expressed circRNAs were involved in protein metabolism, regulation, synapses, and other pathophysiological processes during CeAD-induced stroke. Additionally, various pathways related to inflammation were closely associated with circRNAs. Based on our results, we suggest that the aberrant expression of circRNAs in CeAD may serve as a biomarker for its diagnosis and as a potential therapeutic target.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Dong, Li, Li, Mai, Tan, Yang, Ling and Liu.