The novel immunobiotic Clostridium butyricum S-45-5 displays broad-spectrum antiviral activity in vitro and in vivo by inducing immune modulation

Frontiers in Immunology

Immunology

Jayoung Paek¹  Yeseul Shin¹  Lu Bai¹  Young-Hyo Chang¹  Jong-Soo Lee²  Yebin Seong²  Kiramage Chathuranga²  W. A. Gayan Chathuranga²  Md Bashir Uddin³  Jeong Hwan Shin⁴  Hongik Kim⁵ 

[1] Access to Genetic Resources and Benefit-Sharing (ABS) Research Support Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), Daejeon, Republic of Korea;College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea;College of Veterinary Medicine, Chungnam National University, Daejeon, Republic of Korea;Department of Medicine, Sylhet Agricultural University, Sylhet, Bangladesh;Department of Microbiology and Immunology, University of Texas Medical Branch, Galveston, TX, United States;Department of Laboratory Medicine, Inje University College of Medicine, Busan, Republic of Korea;Research and Development Division, Vitabio, Inc., Daejeon, Republic of Korea;

关键词: Clostridium butyricum;    probiotics;    antiviral activity;    interferon;    influenza virus;   

DOI  :  10.3389/fimmu.2023.1242183

 received in 2023-06-18, accepted in 2023-09-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

Clostridium butyricum is known as a probiotic butyric acid bacterium that can improve the intestinal environment. In this study, we isolated a new strain of C. butyricum from infant feces and evaluated its physiological characteristics and antiviral efficacy by modulating the innate immune responses in vitro and in vivo. The isolated C. butyricum S-45-5 showed typical characteristics of C. butyricum including bile acid resistance, antibacterial ability, and growth promotion of various lactic acid bacteria. As an antiviral effect, C. butyricum S-45-5 markedly reduced the replication of influenza A virus (PR8), Newcastle Disease Virus (NDV), and Herpes Simplex Virus (HSV) in RAW264.7 cells in vitro. This suppression can be explained by the induction of antiviral state in cells by the induction of antiviral, IFN-related genes and secretion of IFNs and pro-inflammatory cytokines. In vivo, oral administration of C. butyricum S-45-5 exhibited prophylactic effects on BALB/c mice against fatal doses of highly pathogenic mouse-adapted influenza A subtypes (H1N1, H3N2, and H9N2). Before challenge with influenza virus, C. butyricum S-45-5-treated BALB/c mice showed increased levels of IFN-β, IFN-γ, IL-6, and IL-12 in serum, the small intestine, and bronchoalveolar lavage fluid (BALF), which correlated with observed prophylactic effects. Interestingly, after challenge with influenza virus, C. butyricum S-45-5-treated BALB/c mice showed reduced levels of pro-inflammatory cytokines and relatively higher levels of anti-inflammatory cytokines at day 7 post-infection. Taken together, these findings suggest that C. butyricum S-45-5 plays an antiviral role in vitro and in vivo by inducing an antiviral state and affects immune modulation to alleviate local and systemic inflammatory responses caused by influenza virus infection. Our study provides the beneficial effects of the new C. butyricum S-45-5 with antiviral effects as a probiotic.

【 授权许可】

Unknown   
Copyright © 2023 Chathuranga, Shin, Uddin, Paek, Chathuranga, Seong, Bai, Kim, Shin, Chang and Lee