Quantitative proteomic analysis and verification identify global protein profiling dynamics in pig during the estrous cycle

Frontiers in Veterinary Science

Veterinary Science

Ying Wang¹  Baohong Li²  Bin Hu³  Fanming Meng³  Sutian Wang³  Jianhao Li³  Haiyun Xin³ 

[1] Guangzhou Customs Technical Center, Guangzhou, China;State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China;State Key Laboratory of Swine and Poultry Breeding Industry, Guangdong Key Laboratory of Animal Breeding and Nutrition, Institute of Animal Science, Guangdong Academy of Agricultural Sciences, Guangzhou, China;Maoming Branch, Guangdong Laboratory for Lingnan Modern Agriculture, Maoming, China;

关键词: sow;    estrus detection;    saliva;    quantitative proteomics;    differentially expressed proteins;    reproduction efficiency;   

DOI  :  10.3389/fvets.2023.1247561

 received in 2023-06-26, accepted in 2023-09-11,  发布年份 2023

来源: Frontiers

【 摘 要 】

The current estrus detection method is generally time-consuming and has low accuracy. As such, a deeper understanding of the physiological processes during the estrous cycle accelerates the development of estrus detection efficiency and accuracy. In this study, the label-free acquisition mass spectrometry was used to explore salivary proteome profiles during the estrous cycle (day −3, day 0, day 3, and day 8) in pigs, and the parallel reaction monitoring (PRM) was applied to verify the relative profiles of protein expression. A total of 1,155 proteins were identified in the label-free analysis, of which 115 were identified as differentially expressed proteins (DEPs) among different groups (p ≤ 0.05). Functional annotation revealed that the DEPs were clustered in calcium ion binding, actin cytoskeleton, and lyase activity. PRM verified the relative profiles of protein expression, in which PHB domain-containing protein, growth factor receptor-bound protein 2, elongation factor Tu, carboxypeptidase D, carbonic anhydrase, and trefoil factor 3 were confirmed to be consistent in both label-free and PRM approaches. Comparative proteomic assays on saliva would increase our knowledge of the estrous cycle in sows and provide potential methods for estrus detection.

【 授权许可】

Unknown   
Copyright © 2023 Xin, Li, Meng, Hu, Wang, Wang and Li.