Diversity of antibiotic resistance genes in soils with four different fertilization treatments

Frontiers in Microbiology

Microbiology

Na Zhang¹  Liang Shao¹  Chunming Li¹  Zhuoran Wang² 

[1] Jilin Bishuiyuan Water Science and Technology Ltd., Co., Changchun, Jilin, China;Key Laboratory of Groundwater Resources and Environment of Ministry of Education, College of New Energy and Environment, Jilin University, Changchun, China;Jilin Provincial Key Laboratory of Water Resources and Environment, College of New Energy and Environment, Jilin University, Changchun, China;National and Local Joint Engineering Laboratory for Petrochemical Contaminated Site Control and Remediation Technology, Jilin University, Changchun, China;

关键词: soil properties;    antibiotic resistance genes;    bacterial community;    fertilization;    horizontal gene transfer;   

DOI  :  10.3389/fmicb.2023.1291599

 received in 2023-09-09, accepted in 2023-10-09,  发布年份 2023

来源: Frontiers

【 摘 要 】

Although the enrichment of resistance genes in soil has been explored in recent years, there are still some key questions to be addressed regarding the variation of ARG composition in soil with different fertilization treatments, such as the core ARGs in soil after different fertilization treatments, the correlation between ARGs and bacterial taxa, etc. For soils after different fertilization treatments, the distribution and combination of ARG in three typical fertilization methods (organic fertilizer alone, chemical fertilizer alone, and conventional fertilizer) and non-fertilized soils were investigated in this study using high-throughput fluorescence quantitative PCR (HT-qPCR) technique. The application of organic fertilizers significantly increased the abundance and quantity of ARGs and their subtypes in the soil compared to the non-fertilized soil, where sul1 was the ARGs specific to organic fertilizers alone and in higher abundance. The conventional fertilizer application also showed significant enrichment of ARGs, which indicated that manure addition often had a more decisive effect on ARGs in soil than chemical fertilizers, and three bacteria, Pseudonocardia, Irregularibacter, and Castllaniella, were the key bacteria affecting ARG changes in soil after fertilization. In addition, nutrient factors and heavy metals also affect the distribution of ARGs in soil and are positively correlated. This paper reveals the possible reasons for the increase in the number of total soil ARGs and their relative abundance under different fertilization treatments, which has positive implications for controlling the transmission of ARGs through the soil-human pathway.

【 授权许可】

Unknown   
Copyright © 2023 Wang, Zhang, Li and Shao.