Prolonging the delivery of influenza virus vaccine improves the quantity and quality of the induced immune responses in mice

Frontiers in Immunology

Immunology

Shuran Gong¹  Kasem Al-Jaawni¹  Jacqueline J. de Vries-Idema¹  Anke Huckriede¹  Martin Beukema¹  Florian Krammer²  Fan Zhou³  Rebecca Jane Cox³ 

[1] Department of Medical Microbiology and Infection Prevention, University of Groningen, University Medical Center Groningen, Groningen, Netherlands;Department of Microbiology, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Center for Vaccine Research and Pandemic Preparedness (C-VaRPP), Icahn School of Medicine at Mount Sinai, New York, NY, United States;Department of Pathology, Molecular and Cell Based Medicine, Icahn School of Medicine at Mount Sinai, New York, NY, United States;Influenza Centre, Department of Clinical Science, University of Bergen, Bergen, Norway;Department of Microbiology, Haukeland University Hospital, Bergen, Norway;

关键词: extended antigen delivery;    influenza;    vaccination;    quality;    quantity;   

DOI  :  10.3389/fimmu.2023.1249902

 received in 2023-06-29, accepted in 2023-09-20,  发布年份 2023

来源: Frontiers

【 摘 要 】

IntroductionInfluenza vaccines play a vital role in protecting individuals from influenza virus infection and severe illness. However, current influenza vaccines have suboptimal efficacy, which is further reduced in cases where the vaccine strains do not match the circulating strains. One strategy to enhance the efficacy of influenza vaccines is by extended antigen delivery, thereby mimicking the antigen kinetics of a natural infection. Prolonging antigen availability was shown to quantitatively enhance influenza virus-specific immune responses but how it affects the quality of the induced immune response is unknown. Therefore, the current study aimed to investigate whether prolongation of the delivery of influenza vaccine improves the quality of the induced immune responses over that induced by prime-boost immunization.MethodsMice were given daily doses of whole inactivated influenza virus vaccine for periods of 14, 21, or 28 days; the control group received prime-boost immunization with a 28 days interval.ResultsOur data show that the highest levels of cellular and humoral immune responses were induced by 28 days of extended antigen delivery, followed by 21, and 14 days of delivery, and prime-boost immunization. Moreover, prolonging vaccine delivery also improved the quality of the induced antibody response, as indicated by higher level of high avidity antibodies, a balanced IgG subclass profile, and a higher level of cross-reactive antibodies.ConclusionsOur findings contribute to a better understanding of the immune response to influenza vaccination and have important implications for the design and development of future slow-release influenza vaccines.

【 授权许可】

Unknown   
Copyright © 2023 Beukema, Gong, Al-Jaawni, de Vries-Idema, Krammer, Zhou, Cox and Huckriede