Understanding blood development and leukemia using sequencing-based technologies and human cell systems

Frontiers in Molecular Biosciences

Molecular Biosciences

Branco M. H. Heuts¹  Joost H. A. Martens² 

[1] Department of Molecular Biology, Faculty of Science, Radboud University, Nijmegen, Netherlands;null;

关键词: transcription factors;    eRNA;    gene regulatory networks;    single-cell sequencing;    iPSC;    functional genomics;    big data;    hematopoiesis;   

DOI  :  10.3389/fmolb.2023.1266697

 received in 2023-07-25, accepted in 2023-09-06,  发布年份 2023

来源: Frontiers

【 摘 要 】

Our current understanding of human hematopoiesis has undergone significant transformation throughout the years, challenging conventional views. The evolution of high-throughput technologies has enabled the accumulation of diverse data types, offering new avenues for investigating key regulatory processes in blood cell production and disease. In this review, we will explore the opportunities presented by these advancements for unraveling the molecular mechanisms underlying normal and abnormal hematopoiesis. Specifically, we will focus on the importance of enhancer-associated regulatory networks and highlight the crucial role of enhancer-derived transcription regulation. Additionally, we will discuss the unprecedented power of single-cell methods and the progression in using in vitro human blood differentiation system, in particular induced pluripotent stem cell models, in dissecting hematopoietic processes. Furthermore, we will explore the potential of ever more nuanced patient profiling to allow precision medicine approaches. Ultimately, we advocate for a multiparameter, regulatory network-based approach for providing a more holistic understanding of normal hematopoiesis and blood disorders.

【 授权许可】

Unknown   
Copyright © 2023 Heuts and Martens.